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http://www.addgene.org/63850

Species: Synthetic
Genetic Insert: Theo45 theophylline riboswitch
Vector Backbone Description: Vector Backbone:pFTV1; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26621913

Proper citation: RRID:Addgene_63850 Copy   

•   Source: Addgene

http://www.addgene.org/63901

Species: Homo sapiens
Genetic Insert: TT Fab
Vector Backbone Description: Backbone Size:3500; Vector Backbone:pAra; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: Provided with the pComb3H system for arabinose induced expression of soluble protein with detection tags. The “TT” refers to the human Fab to tetanus toxin which can be used as a protein expression control. Alternatively, one could upgrade to the pComb3X system, also available on Addgene, which already contains tags. Select reference: Cary, S.P.; Lee, J.; Wagenknecht, R.; Silverman, G.J. (2000) Characterization of Superantigen-Induced Clonal Deletion with a Novel Clan III-Restricted Avian Monoclonal Antibody: Exploiting Evolutionary Distance to Create Antibodies Specific for a Conserved VH Region Surface. J. Immun., 164, 4730-4741.

Proper citation: RRID:Addgene_63901 Copy   

•   Source: Addgene

http://www.addgene.org/64026

Vector Backbone Description: Backbone Marker:DNA 2.0; Backbone Size:3792; Vector Backbone:pJ344; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26133196

Proper citation: RRID:Addgene_64026 Copy   

•   Source: Addgene

http://www.addgene.org/50548

Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Note that Addgene NGS identified a nonsense mutation in the CcaS insert

Proper citation: RRID:Addgene_50548 Copy   

•   Source: Addgene

http://www.addgene.org/50552

Species: Synthetic
Genetic Insert: Cph8
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1995; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181
Comments: Encodes the Cph8 (Cph1/EnvZ fusion) light sensor kinase/phosphatase downstream of the pLTetO-1 promoter. Requires pPLPCB(S)t or some other source of TetR Requires PCB to function Does NOT include the response regulator.

Proper citation: RRID:Addgene_50552 Copy   

•   Source: Addgene

http://www.addgene.org/50551

Species: Synechocystis sp. 6803
Genetic Insert: CcaS
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:1900; Vector Backbone:pProTet.E333; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24608181

Proper citation: RRID:Addgene_50551 Copy   

•   Source: Addgene

http://www.addgene.org/50593

Species: Synthetic
Genetic Insert: T1, gRNA scaffold, OsU3t plus, TaU3p, T2
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517
Comments: Please note that this plasmid runs as a dimer (>7kb) or multimer.

Proper citation: RRID:Addgene_50593 Copy   

•   Source: Addgene

http://www.addgene.org/50595

Species: Synthetic
Genetic Insert: T3, gRNA scaffold, U6-26t plus, OsU3p, T4
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517

Proper citation: RRID:Addgene_50595 Copy   

•   Source: Addgene

http://www.addgene.org/50590

Species: Synthetic
Genetic Insert: T1, U626t plus, U629p, T2
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517

Proper citation: RRID:Addgene_50590 Copy   

•   Source: Addgene

http://www.addgene.org/50592

Species: Synthetic
Genetic Insert: T3, gRNA scaffold, U6-1tplus, U6-26p, T4
Vector Backbone Description: Vector Backbone:pGWC; Vector Types:Plant Expression, CRISPR, PCR template; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25432517

Proper citation: RRID:Addgene_50592 Copy   

•   Source: Addgene

http://www.addgene.org/55179

Species: Synthetic
Genetic Insert: His10
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers: fwd: TGCCACCTGACGTCTAAG rev: ATTACCGCCTTTGAGTGA

Proper citation: RRID:Addgene_55179 Copy   

•   Source: Addgene

http://www.addgene.org/55183

Species: Synthetic
Genetic Insert: cMyc tag
Vector Backbone Description: Backbone Marker:iGEM; Backbone Size:2070; Vector Backbone:pSB1C3; Vector Types:Synthetic Biology, Bacillus BioBrick Box; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24295448
Comments: For sequencing of insert, use the following primers: fwd: TGCCACCTGACGTCTAAG rev: ATTACCGCCTTTGAGTGA

Proper citation: RRID:Addgene_55183 Copy   

•   Source: Addgene

http://www.addgene.org/61920

Genetic Insert: none
Vector Backbone Description: Vector Backbone:none; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:21925267
Comments: Genotype = cyo ilvE avtA aspC hisG argH metA lysA Precursor strain = ML31 Selective amino acid labeling (and/or requirement) = (Ala#), Ile, Leu*, Tyr#, Val, His, Arg, Met, Lys ML40(K1) contains an extra pACYC plasmid carrying the cat cassette plus extra tRNA genes (argU, ileY, leuW) from Agilent Technologies Inc. *In the presence of 0.4-1 mM Tyr, tyrB is repressed and Leu is required for growth in minimal medium. This strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign genes [Methods 55, 370-378 (2011)]. #In the presence of 0.4-1 mM Tyr, tyrB is repressed and Tyr is required for growth in minimal medium. Under these conditions, this strain can also be used for selective labeling of the input Tyr and/or Ala label(s), although minor diffusion of the input label(s) can occur. Note that this strategy can only be applicable for a short-term cultivation but not suitable for a long-term cultivation for heterologous expression of foreign geness [Methods 55, 370-378 (2011)]. Each target gene was deleted from the chromosome of C43 (DE3) E. coli strain using λ-Red recombination system. Supplemental documents contain a list of PCR primers used for verification of each knocked-out gene as well as an image showing PCR results for this strain.

Proper citation: RRID:Addgene_61920 Copy   

•   Source: Addgene

http://www.addgene.org/61805

Vector Backbone Description: Backbone Marker:Bacillus Genetic Stock Center; Backbone Size:4217; Vector Backbone:pNW33N; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22578246

Proper citation: RRID:Addgene_61805 Copy   

•   Source: Addgene

http://www.addgene.org/62329

Vector Backbone Description: Backbone Marker:Structural Genomics Consortium; Backbone Size:8362; Vector Backbone:pACYC-LIC+; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol

Proper citation: RRID:Addgene_62329 Copy   

•   Source: Addgene

http://www.addgene.org/62655

Genetic Insert: Cas9 nuclease
Vector Backbone Description: Vector Backbone:pdCas9-bacteria; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:26463009
Comments: Please note that this plasmid runs as a dimer (>13kb). While this may reduce DNA yield, the plasmid still functions as expected.

Proper citation: RRID:Addgene_62655 Copy   

•   Source: Addgene

http://www.addgene.org/48107

Vector Backbone Description: Backbone Marker:Dieter Haas; Backbone Size:8270; Vector Backbone:pME6010; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:23995928

Proper citation: RRID:Addgene_48107 Copy   

•   Source: Addgene

http://www.addgene.org/48656

Species: Synthetic
Genetic Insert: Bacterial TD crRNA to prototspacer B
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762

Proper citation: RRID:Addgene_48656 Copy   

•   Source: Addgene

http://www.addgene.org/48655

Species: Synthetic
Genetic Insert: Bacterial TD crRNA to prototspacer A
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762

Proper citation: RRID:Addgene_48655 Copy   

•   Source: Addgene

http://www.addgene.org/48654

Species: Synthetic
Genetic Insert: Bacterial ST1 crRNA to prototspacer B
Vector Backbone Description: Vector Backbone:p15A-cat; Vector Types:CRISPR; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24076762

Proper citation: RRID:Addgene_48654 Copy   

•   Source: Addgene