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Species: Homo sapiens
Genetic Insert: PCSK5
Vector Backbone Description: Backbone Marker:Iain Fraser, Addgene plasmid #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_232449 Copy
Species: Homo sapiens
Genetic Insert: PCSK5
Vector Backbone Description: Backbone Marker:Iain Fraser, Addgene plasmid #25752; Vector Backbone:pEN_TTmiRC2; Vector Types:Gateway Cloning; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_232448 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238325 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238323 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238321 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238328 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238326 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238312 Copy
Species: Homo sapiens
Genetic Insert: TRPML1
Vector Backbone Description: Backbone Marker:Geneva Biotech; Backbone Size:4618; Vector Backbone:pACEMam2; Vector Types:Mammalian Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238303 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: VIP1
Vector Backbone Description: Vector Backbone:pBB3; Vector Types:Insect Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_238319 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Size:5888; Vector Backbone:pKEK2201; Vector Types:Sucrose counter-selection allelic exchange vector; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_252056 Copy
Species:
Genetic Insert: Transposon donor V1 encoding acrVIA1
Vector Backbone Description: Vector Backbone:pHERD30T; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_250983 Copy
Species: BxbI phage
Genetic Insert: BxbI phage integrase
Vector Backbone Description: Backbone Size:3740; Vector Backbone:pSEVA6213; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_227669 Copy
Species: Synthetic
Genetic Insert: msfGFP
Vector Backbone Description: Backbone Size:4546; Vector Backbone:pBAMD1-6; Vector Types:Bacterial Expression; Bacterial Resistance:Gentamicin
References:
Comments:
Proper citation: RRID:Addgene_227665 Copy
Species:
Genetic Insert:
Vector Backbone Description: Backbone Marker:Ichiro Matsumura, Addgene plasmid #26702; Backbone Size:2515; Vector Backbone:pBAV1K-T5-gfp; Vector Types:Unspecified; Bacterial Resistance:Gentamicin
References:
Comments: Use Gentamicin 15 µg/mL in E. coli, Gentamicin 30 µg/mL in Synechococcus sp. PCC 7002
Grow at 30°C in E. coli, grow at 37°C in Synechococcus sp. PCC 7002
For increased transformation into PCC 7002, miniprep this plasmid from E.coli strain DHIP1.
DHIP1 deposited under the following paper:
Kamoku C, Nielsen DR. Highly Iterated Palindrome 1 (HIP1) sequence improves Synechococcus sp. PCC 7002 transformation efficiencies in a homology- and methylation-dependent manner. Published online September 10, 2024:2024.09.10.611283. doi:10.1101/2024.09.10.611283
Please visit https://doi.org/10.1101/2024.07.31.606084 for bioRxiv preprint.
Proper citation: RRID:Addgene_226658 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229955 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229954 Copy
Species: Homo sapiens
Genetic Insert: ActBT2ATetP2AP - H
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229972 Copy
Species: Homo sapiens
Genetic Insert: ActBT2ATetP2AP - mAGH
Vector Backbone Description: Vector Backbone:pBIG; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229973 Copy
Species:
Genetic Insert:
Vector Backbone Description: Vector Backbone:pLIB; Vector Types:Mammalian Expression, CRISPR; Bacterial Resistance:Gentamicin
References:
Comments: Please visit https://doi.org/10.1101/2024.04.18.590029 for bioRxiv preprint.
Proper citation: RRID:Addgene_229956 Copy
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