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Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Comments: Strain description: CsrA system knockout
Chromosomal changes: ΔCsrA, ΔCsrB, ΔCsrC, ΔglgCAP, ΔpgaABCD, +LacIq at galK
Proper citation: RRID:Addgene_37980 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Proper citation: RRID:Addgene_37976 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: ΔDam
Chromosomal changes: ΔDam
Proper citation: RRID:Addgene_37977 Copy
Genetic Insert: See comments
Vector Backbone Description: Vector Backbone:N/A; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:17220888
Comments: Host strain for pHL32B
Strain description: Δ(Dam+OxyR)
Chromosomal changes: Δ(Dam+OxyR)
Proper citation: RRID:Addgene_37979 Copy
Genetic Insert: Bxb1-Excisionase
Vector Backbone Description: Backbone Size:2800; Vector Backbone:J64100; Vector Types:Synthetic Biology, BioBrick cloning vector; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:22615351
Comments: In the reset circuit, PBAD controls expression of a polycistron encoding excisionase with a strong RBS followed by Bxb1 integrase with a GTG start codon to decrease its translation rate
and increase the excisionase to integrase ratio. This construct was cloned in a plasmid
with a regulated ColE1 origin of replication (J64100, 50-70 copies, chloramphenicol resistance).
Proper citation: RRID:Addgene_38207 Copy
Species: Homo sapiens
Genetic Insert: AASDHPPT
Vector Backbone Description: Backbone Marker:SGC; Vector Backbone:pCOEX-1; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Comments: http://www.thesgc.org/structures/2BYD/
Proper citation: RRID:Addgene_39192 Copy
Genetic Insert: encodes methylases AvaiM and Eco47iiM that target sites subject to restriction in Anabaena sp. strain PCC 7120
Vector Backbone Description: Vector Backbone:pRL542; Vector Types:; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:3148842
Comments: MobColK, M.AvaI, M.Eco47II
Please see attached map for more information on how this vector was constructed and cloning details.
Proper citation: RRID:Addgene_58495 Copy
Genetic Insert: rfp
Vector Backbone Description: Backbone Size:8144; Vector Backbone:pKT230; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:24219429
Proper citation: RRID:Addgene_59772 Copy
Species: Other
Genetic Insert: Ham1
Vector Backbone Description: Vector Backbone:pSALECT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25790188
Proper citation: RRID:Addgene_59706 Copy
Species: Mus musculus
Genetic Insert: SupH
Vector Backbone Description: Vector Backbone:pSALECT; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:25790188
Proper citation: RRID:Addgene_59710 Copy
Species: Other
Genetic Insert: Marchantia polymorpha petB 5'UTR
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34097383
Proper citation: RRID:Addgene_163933 Copy
Species: Other
Genetic Insert: Nicotiana tabacum psba terminator
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Proper citation: RRID:Addgene_163932 Copy
Species: Other
Genetic Insert: aadA gene for spectinomycin resistance
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Proper citation: RRID:Addgene_163935 Copy
Species: Other
Genetic Insert: Marchantia polymorpha chloroplast DNA-dependent RNA polymerase operon promoter (rpo) (genome position: 5595-5739)
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:32163700
Proper citation: RRID:Addgene_163951 Copy
Species: Other
Genetic Insert: Nicotiana tabacum psbA promoter
Vector Backbone Description: Vector Backbone:pUAP4; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34097383
Proper citation: RRID:Addgene_163950 Copy
Vector Backbone Description: Backbone Size:5352; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33271061
Comments: Reference for vector backbone.
Guzman, L. M., Belin, D., Carson, M. J., & Beckwith, J. (1995). Tight regulation, modulation, and high-level expression by vectors containing the arabinose PBAD promoter. Journal of Bacteriology, 177(14), 4121–4130.
Proper citation: RRID:Addgene_164263 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-3
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164860 Copy
Species: Leptotrichia buccalis
Genetic Insert: AcrVIA7
Vector Backbone Description: Backbone Size:4010; Vector Backbone:pBAD33; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: The anti-CRISPR protein AcrVIA7 was codon-optimized for E. coli for optimal expression.
Proper citation: RRID:Addgene_164864 Copy
Species: Bergeyella zoohelcum
Genetic Insert: BzCas13b-gRNA-1
Vector Backbone Description: Backbone Size:4215; Vector Backbone:pACYC184; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34694607
Comments: Spacer sequence is designed to target the mRNA of deGFP.
Proper citation: RRID:Addgene_164858 Copy
Species: Synthetic
Genetic Insert: CT81 gene circuit
Vector Backbone Description: Backbone Size:9311; Vector Backbone:pMMB206; Vector Types:Bacterial Expression; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:33558556
Comments: pMMB206 backbone is very low copy, <10 copies.
Proper citation: RRID:Addgene_165411 Copy
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