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Species: Aequorea victoria
Genetic Insert: synthetic sfYFP (codon usage adapted to P.putida KT2440)
Vector Backbone Description: Backbone Marker:Dammeyer et al. 2013; Vector Backbone:pTD-CTwinStrep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:23687945
Comments: This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates.
The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long
Proper citation: RRID:Addgene_45943 Copy
Species: Aequorea victoria
Genetic Insert: synthetic eYFP (codon usage adapted to E.coli K12)
Vector Backbone Description: Backbone Marker:Dammeyer et al. 2013; Vector Backbone:pTD-CTwinStrep; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:23687945
Comments: This plasmid was tested in the Gram-negative soil bacterium Pseudomonas putida KT2440 and Escherichia coli K12 and is especially suited for protein production, affinity purification, protein complex copurification with SPINE (Strep Protein Interaction Experiments) or (co-)localization studies. Due to the broad host range of the RK2 origin of replication, the plasmid facilitates experimental verification of hypothetical proteins and protein production yield assessment in different expression hosts possibly including new isolates.
The Supplementary Table S1 in the following publication lists approximately 30 strains in which the RK2 origin of replication should be functional. Silva-Rocha et al., The Standard European Vector Architecture (SEVA): a coherent platform for the analysis and deployment of complex prokaryotic phenotypes. Nucleic Acids Research 2013, 41:D666-675. http://nar.oxfordjournals.org/content/41/D1/D666.long
Proper citation: RRID:Addgene_45942 Copy
Vector Backbone Description: Vector Backbone:genome; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:22822084
Comments: selection using 10-15 micrograms/mL strep.
It is MalE deficient, which enables maltose complementation. For the assay, a particular construct is transformed into MM39 and grown on maltose minimal media to confirm proper membrane orientation and integration.
Proper citation: RRID:Addgene_42894 Copy
Species: Rattus norvegicus
Genetic Insert: rApoI
Vector Backbone Description: Vector Backbone:None; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:29991261
Comments: Esherichia coli strain that expresses a deactivated variant of MutaT7, a fusion of rat APOBEC1 and the T7 RNA polymerase. Genotype: DH10B Δung ΔmotAB ΔcsgABCDEFG [PlacI<>Ptac] Δ(araA-leu)7697::[PA1lacO-Tenth drApo1-T7]
Proper citation: RRID:Addgene_156461 Copy
Species: E. coli
Genetic Insert: BJ5183 bacterial cells
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:9482916
Comments: This is a bacterial strain - not a plasmid. Select with 30 ug/mL streptomycin.
Electrocompetent BJ5183 cells are used to carry out the recombination between the shuttle vector (pShuttle, pShuttle-CMV, pAdTrack, or pAdTrack-CMV) and the adenoviral vector (pAdEasy1 or pAdEasy2).
Proper citation: RRID:Addgene_16398 Copy
Species: SARS-CoV-2 isolate Wuhan-Hu
Genetic Insert: NSP7
Vector Backbone Description: Backbone Size:4000; Vector Backbone:pCDF Duet; Vector Types:Bacterial Expression; Bacterial Resistance:Streptomycin
Defining Citation: PMID:32783916
Comments: Please visit https://doi.org/10.1101/2020.07.08.194084 for bioRxiv preprint.
Proper citation: RRID:Addgene_159092 Copy
Species: Phaeodactylum tricornutum
Genetic Insert: Phaeodactylum U6 promoter
Vector Backbone Description: Backbone Size:2817; Vector Backbone:pCR8/GW/TOPO; Vector Types:; Bacterial Resistance:Streptomycin
Defining Citation: PMID:34566902
Comments: For Golden Gated assembly.
Please visit https://www.biorxiv.org/content/early/2018/10/17/444109 for bioRxiv preprint.
Proper citation: RRID:Addgene_104895 Copy
Vector Backbone Description: Backbone Marker:Esteban Martínez-García and Víctor de Lorenzo; Backbone Size:3168; Vector Backbone:pEMG; Vector Types:Bacterial Expression, Synthetic Biology; Bacterial Resistance:Streptomycin
Defining Citation: PMID:30861315
Proper citation: RRID:Addgene_122088 Copy
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