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Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18242 Copy
Species: Other
Genetic Insert: Reverse compliment of antiRTA3kb
Vector Backbone Description: Vector Backbone:pCDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182601 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18244 Copy
Species: Other
Genetic Insert: Reverse compliment of T15
Vector Backbone Description: Vector Backbone:pCDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182603 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18246 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18247 Copy
Species: Other
Genetic Insert: antiRTA3kb
Vector Backbone Description: Vector Backbone:pCDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182600 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18248 Copy
Species: Rattus norvegicus
Genetic Insert: rat Scn2a gRNA and 3xV5 donor DNA
Vector Backbone Description: Vector Backbone:PX552; Vector Types:AAV, CRISPR; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35672149
Proper citation: RRID:Addgene_182566 Copy
Species: Other
Genetic Insert: Reverse compliment of T61
Vector Backbone Description: Vector Backbone:pCDNA3.1 (-) ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182609 Copy
Species: Other
Genetic Insert: Reverse compliment of antiRTA1kb (pOME0242)
Vector Backbone Description: Vector Backbone:pCDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182605 Copy
Species: Other
Genetic Insert: T61
Vector Backbone Description: Vector Backbone:pCDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182608 Copy
Species: Synthetic
Genetic Insert: BSD
Vector Backbone Description: Backbone Marker:Alfred L. Fisher; Vector Backbone:pMOD4-galK-G; Vector Types:Bacterial Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: PCR amplify around the 5' T2A and 3' FRT site, making sure to add a PacI site at the 3' of the FRT site.
As suggested in the plasmid name, due to a PCR error, the rpsL open reading frame has a 1-bp deletion that results in a frame shift and as a consequence premature stop codons. Despite this, the downstream BSD open reading frame is translated from the bicistronic RNA in E. coli without problems. These mean that the mutated rpsL cannot be used for streptomycin counterselection, but the unmutated BSD can and was successfully used several times to confer blasticidin resistance for selection after recombineering.
Proper citation: RRID:Addgene_182338 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18253 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18254 Copy
Species: Other
Genetic Insert: HHV8 antiPAN
Vector Backbone Description: Vector Backbone:pCDNA3.1 (+); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182615 Copy
Species: Synthetic
Genetic Insert: T2A-td-sfGFP-FNF
Vector Backbone Description: Backbone Marker:Hyung-song Nam, Capecchi laboratory; Vector Backbone:Custom; Vector Types:Mammalian Expression, Mouse Targeting, FLP / FRT; Bacterial Resistance:Ampicillin
Comments: Release the insert with PmeI and PacI digest.
Proper citation: RRID:Addgene_182339 Copy
Species: Other
Genetic Insert: Reverse compliment HHV8 TO_7 control
Vector Backbone Description: Vector Backbone:pCDNA3.1 (-); Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Proper citation: RRID:Addgene_182614 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18259 Copy
Species: Bacteriophage T4
Genetic Insert: T4 Lysozyme
Vector Backbone Description: Backbone Size:5000; Vector Backbone:pHS1403; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The BamHI site is 27 bp from the ATG and the HindIII site is 115 bp from the stop.
Proper citation: RRID:Addgene_18240 Copy
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