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Species: Synthetic
Genetic Insert: VHH template2-3XMyc-Spacer
Vector Backbone Description: Backbone Size:2561; Vector Backbone:pBx1; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34535642
Proper citation: RRID:Addgene_176209 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 5' homology for integration site XI-1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176166 Copy
Species: E. coli
Genetic Insert: mCherry-eDHFR
Vector Backbone Description: Backbone Marker:Dr. Eugene Makeyev; Backbone Size:-1; Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904910
Comments: Please note that there are minor differences between the Addgene NGS result and the depositor's sequence. These differences do not alter plasmid function.
Proper citation: RRID:Addgene_176201 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-1
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176167 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcD
Vector Backbone Description: Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34780538
Comments: encodes (pelC signal sequence)-His10-GfcD(22-698)
Proper citation: RRID:Addgene_176200 Copy
Species: Saccharomyces cerevisiae
Genetic Insert: 3' homology for integration site XI-2
Vector Backbone Description: Vector Backbone:pYTK001; Vector Types:Synthetic Biology; Bacterial Resistance:Chloramphenicol
Defining Citation: PMID:34860007
Comments: Constructed using the Yeast Modular Cloning Toolkit (Lee et al. ACS Synth Biol. 2015). Homologous region amplified from EasyClone-MarkerFree toolkit (Jessop-Fabre et al. Biotechnol J. 2016).
Proper citation: RRID:Addgene_176169 Copy
Species: Homo sapiens
Genetic Insert: mCherry-INBox-eDHFR
Vector Backbone Description: Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904910
Proper citation: RRID:Addgene_176202 Copy
Species: Mus musculus
Genetic Insert: delFasL
Vector Backbone Description: Backbone Size:0; Vector Backbone:EF.CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:12788657
Comments: The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP, with a unique EcoRV site available to insert a gene of interest under the control of the EF promoter. Noncleavable mouse delFasL cDNA was constructed using PCR cloning to delete the exon 2 region, which contains the metalloproteinase cleavage site. The delFasL gene was then cloned into the EcoRV site of EF.CMV.GFP to make the EF.delFasL-CMV.GFP LV.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17620 Copy
Species: Homo sapiens
Genetic Insert: MCAK-mCherry-eDHFR
Vector Backbone Description: Vector Backbone:pEM791; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33904910
Proper citation: RRID:Addgene_176204 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Marker:Linzhao Cheng Lab; Backbone Size:8200; Vector Backbone:EF.v-CMV.GFP; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
The CMV promoter was inserted immediately upstream of GFP in the EF.GFP LV to make the intermediate LV, EF.V-CMV.GFP. hICN1 was cloned downstream of the EF-1a promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17623 Copy
Species: Homo sapiens
Genetic Insert: ICN1
Vector Backbone Description: Backbone Size:11500; Vector Backbone:cFUGW; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:16513761
Comments: Addgene sequence shows that there is an A1902S mutation in Notch1 relative to NCBI sequences. This mutation is not known to affect function.
hICN1 driven by the EF-1a promoter and a GFP reporter driven by the ubiquitin C (Ubc) promoter.
Recombinant LVs were produced by transient transfection of the transducing vector into 293T cells with two packaging vectors: pMD.G, a plasmid expressing the VSV-G envelope gene, and pCMVDeltaR8.91, a plasmid expressing the HIV-1 gag/pol, tat, and rev genes.
Proper citation: RRID:Addgene_17626 Copy
Genetic Insert: UBC promoter - GFP - PGK promoter - Hygro
Vector Backbone Description: Backbone Size:0; Vector Backbone:Duet011; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:17158240
Comments: Lentiviral dual promoter/dual-gene expression vector is dubbed Duet (for Dual-gene, excisable transgene).
Proper citation: RRID:Addgene_17627 Copy
Species: Francisella novicida
Genetic Insert: humanized fnCas12a
Vector Backbone Description: Vector Backbone:pUC19; Vector Types:CRISPR, Luciferase, Synthetic Biology, Expression in microalgae Nannochloropsis oceanica; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34793143
Proper citation: RRID:Addgene_176239 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcD
Vector Backbone Description: Backbone Marker:from Dr Frank Collart; Vector Backbone:pBH31; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34780538
Comments: encodes (pelC signal sequence)-His6-GfcD(22-698)
Proper citation: RRID:Addgene_176199 Copy
Vector Backbone Description: Vector Backbone:Addgene# 136269; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35323942
Proper citation: RRID:Addgene_176234 Copy
Species: Homo sapiens
Genetic Insert: spCas9 and polymerase exo domain fusion
Vector Backbone Description: Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35323942
Proper citation: RRID:Addgene_176236 Copy
Species: Homo sapiens
Genetic Insert: spCas9 and polymerase exo domain fusion
Vector Backbone Description: Vector Backbone:pspCas9-3'UTR-Tetra-COM-Vector; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:35323942
Proper citation: RRID:Addgene_176237 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcBCD
Vector Backbone Description: Backbone Marker:from Dr Shoshi Altuvia; Vector Backbone:pSA10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34780538
Comments: Insert is a contiguous set of genes gfcB, gfcC, gfcD. All 3 proteins are expressed with their native signal sequences
Proper citation: RRID:Addgene_176193 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcBCD
Vector Backbone Description: Backbone Marker:from Dr Shoshi Altuvia; Vector Backbone:pSA10; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34780538
Comments: Insert is a contiguous set of genes gfcB, gfcC, gfcD. All 3 proteins are expressed with their native signal sequences. Derived from plasmid pSA10(gfcBCD) [Addgene #176193] by mutagenesis to include a 6xHis tag on the C-term of GfcB.
Proper citation: RRID:Addgene_176194 Copy
Species: E. coli E2348/69 O127:H6
Genetic Insert: gfcC
Vector Backbone Description: Vector Backbone:pMCSG7; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34780538
Comments: encodes His6-(x)8-TeV-GfcC(17-214)
Proper citation: RRID:Addgene_176197 Copy
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