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Species: Mus musculus
Genetic Insert: dnPKA
Vector Backbone Description: Backbone Size:4100; Vector Backbone:CS2+; Vector Types:Xenopus Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:8812120
Comments: Insert: dominant negative Protein Kinase A-bright green flourescent protein fusion protein.
To make sense RNA, for expression, cut with Not1 and transcribe with SP6.
Plasmid History:
Constructed by Anne Ungar in 1996.
Citation: Ungar and Moon (1996)
Developmental Biology 178:186-191.
Proper citation: RRID:Addgene_16716 Copy
Species: Escherichia coli, Neonothopanus nambi
Genetic Insert: caffeic acid cycle genes
Vector Backbone Description: Backbone Size:4839; Vector Backbone:Level2-like; Vector Types:Plant Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:32341562
Comments: Encodes 5 transcription units for expression in plants: Kanamycin resistance cassette, Neonothopanus nambi hispidin synthase, Neonothopanus nambi hispidin-3-hydroxylase, Neonothopanus nambi luciferase, Neonothopanus nambi caffeoylpiruvate hydrolase
The depositing lab does not recommend this plasmid for most applications, and recommend using pNK3071 instead (https://www.addgene.org/219755/)
Proper citation: RRID:Addgene_167156 Copy
Species: Neonothopanus nambi
Genetic Insert: p35s-nnHispS-ocsT
Vector Backbone Description: Backbone Size:4372; Vector Backbone:Level1-like (position 2); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32341562
Proper citation: RRID:Addgene_167151 Copy
Species: Synthetic
Genetic Insert: SNAPf-tag
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167271 Copy
Species: Synthetic
Genetic Insert: SNAP-tag
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167270 Copy
Species: Neonothopanus nambi
Genetic Insert: p35s-nnH3H-ocsT
Vector Backbone Description: Backbone Size:4372; Vector Backbone:Level1-like (position 3); Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32341562
Proper citation: RRID:Addgene_167152 Copy
Species: Synthetic
Genetic Insert: SNAP-tag
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET51b(+); Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:34339177
Comments: Please visit https://www.biorxiv.org/content/10.1101/2021.04.13.439540v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167273 Copy
Species: HIV-1
Genetic Insert: gag_tat_env
Vector Backbone Description: Backbone Marker:IDT; Vector Backbone:pUCIDT Amp; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33948574
Comments: This plasmid has partial sequences for 3 different HIV-1 clade B genes. This plasmid is made by digesting the Seattle_IPDA_control_1_001 with NsiI, ligating the result and digesting again with PacI. partial sequences for 3 HIV-1 clade B targets in the gag, tat and env genes
Proper citation: RRID:Addgene_167349 Copy
Species: HIV-1
Genetic Insert: pol_gag_tat
Vector Backbone Description: Backbone Marker:IDT; Vector Backbone:pUCIDT Amp; Vector Types:Other; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33948574
Comments: This plasmid has partial sequences for 3 different HIV-1 clade B genes. This plasmid is made by cutting out one of the targets from the Seattle_IPDA_control_1_001 plasmid using SalI-HF. partial sequences for 4 HIV-1 clade B targets: 5'pol, 3'pol, tat and gag
Proper citation: RRID:Addgene_167348 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6227; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619770
Comments: The mKate2 gene was initially obtained from the commercially available plasmid: pLenti-GIII-CMV-TFAM-RFP-2A-Puro. Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint
Proper citation: RRID:Addgene_167335 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6227; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619770
Comments: The L6 mutant TFAM gene was originally obtained from Addgene Plasmid #60012. The mKate2 gene was initially obtained from the commercially available plasmid: pLenti-GIII-CMV-TFAM-RFP-2A-Puro. Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint
Proper citation: RRID:Addgene_167332 Copy
Species: Homo sapiens
Genetic Insert: Progerin
Vector Backbone Description: Backbone Size:7208; Vector Backbone:pCDH_blast_NCS_Nard; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619770
Comments: Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint
Proper citation: RRID:Addgene_167339 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6227; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619770
Comments: The mKate2 gene was initially obtained from the commercially available plasmid: pLenti-GIII-CMV-TFAM-RFP-2A-Puro. Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint
Proper citation: RRID:Addgene_167338 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6227; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619770
Comments: The mKate2 gene was initially obtained from the commercially available plasmid: pLenti-GIII-CMV-TFAM-RFP-2A-Puro. Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint
Proper citation: RRID:Addgene_167337 Copy
Species: Homo sapiens
Genetic Insert: shRNA hsDDX58
Vector Backbone Description: Backbone Size:7026; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33627873
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.31.929075v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167293 Copy
Species: Homo sapiens
Genetic Insert: TFAM
Vector Backbone Description: Backbone Marker:Addgene; Backbone Size:6227; Vector Backbone:pCDH-CMV-MCS; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33619770
Comments: The TFAM and mKate2 genes were initially obtained from the commercially available plasmid: pLenti-GIII-CMV-TFAM-RFP-2A-Puro. Please visit https://www.biorxiv.org/content/10.1101/822858v4 for BioRxiv preprint
Proper citation: RRID:Addgene_167331 Copy
Species: Homo sapiens
Genetic Insert: BAK
Vector Backbone Description: Vector Backbone:PX458; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:33627873
Comments: Please visit https://www.biorxiv.org/content/10.1101/2020.01.31.929075v1 for bioRxiv preprint.
Proper citation: RRID:Addgene_167295 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5045
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167365 Copy
Species: M. smegmatis
Genetic Insert: MSMEG_5058
Vector Backbone Description: Vector Backbone:pMSR:Dendra; Vector Types:Bacterial Expression, Integrating Mycobacterial Expression Vector attL5. T7 expression in E. coli; Bacterial Resistance:Apramycin
Defining Citation: PMID:33653882
Comments: 5' cloning site: AseI (destroyed), 3' cloning site: HindIII (not destroyed)
Proper citation: RRID:Addgene_167369 Copy
Species: Other
Genetic Insert: A3Bctd-Cas9-UDG-T2A-AP lyase
Vector Backbone Description: Vector Backbone:pJIT163; Vector Types:Plant Expression; Bacterial Resistance:Ampicillin
Defining Citation: PMID:32601432
Proper citation: RRID:Addgene_167360 Copy
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