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Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58172 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58174 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58173 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58176 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58180 Copy
Species: Escherichia coli
Genetic Insert: hygromycin B phosphotransferase
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Proper citation: RRID:Addgene_58223 Copy
Species: Streptomyces sp.
Genetic Insert: phosphinothricin acetyl transferase
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Proper citation: RRID:Addgene_58226 Copy
Vector Backbone Description: Vector Backbone:pSNAPf-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag TBRII contains the H385N mutation .
Proper citation: RRID:Addgene_58187 Copy
Species: Magnaporthe grisea
Genetic Insert: ILV2
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Please note that the actual sequence of the SUR gene in this plasmid differs from the Genbank reference sequence AF013601 by a G120V mutation in the ILV2 locus. G120V does not contribute to the sulfonyl urea resistance per se.
Proper citation: RRID:Addgene_58222 Copy
Species: Magnaporthe grisea
Genetic Insert: ILV2
Vector Backbone Description: Vector Backbone:pFGL815; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Comments: Reference:
Sweigard J, Chumley F, Carroll A, Farrall L, Valent B (1997) A series of vectors for fungal transformation. Fungal Genet Newsl 44: 52-53
Please note that the actual sequence of the SUR gene in this plasmid differs from the Genbank reference sequence AF013601 by a G120V mutation in the ILV2 locus. G120V does not contribute to the sulfonyl urea resistance per se.
Proper citation: RRID:Addgene_58221 Copy
Species: Homo sapiens
Genetic Insert: Clathrin
Vector Backbone Description: Vector Backbone:pSNAPf; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag.
Proper citation: RRID:Addgene_58190 Copy
Species: Homo sapiens
Genetic Insert: LaminA
Vector Backbone Description: Vector Backbone:pSNAPf-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag.
Proper citation: RRID:Addgene_58193 Copy
Species: Homo sapiens
Genetic Insert: PDHA1
Vector Backbone Description: Vector Backbone:pSNAPf-N1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: With SNAP tag.
Proper citation: RRID:Addgene_58195 Copy
Species: Synthetic
Genetic Insert: pea APX W41F
Vector Backbone Description: Backbone Size:2226; Vector Backbone:pZE; Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24803433
Comments: This plasmid encodes the W41F mutant of pea ascorbate peroxidase (APX). The W41F mutation improves enzyme activity. APX W41F is a constitutive homodimer, unlike "APEX", so APX W41F should not be used as a fusion to proteins that are sensitive to dimeric tags.
This plasmid encodes APX W41F under a constitutive promoter, and the promoter is flanked by TetR operator sites. When the plasmid is introduced into cells constitutively expressing the TetR protein, such as the GM1655 Pro cells, then the TetR protein binds to the promoter and suppresses transcription. When a tetracycline drug, such as anhydrotetracycline, is added, the TetR protein is displaced from the promoter, allowing transcription of the APX W41F gene. When the plasmid is introduced into bacterial strains that do not encode a copy of the TetR repressor, expression of APX W41F is constitutive. Any bacterial strain can be used for plasmid propagation.
The pZE plasmid system was developed by Lutz and Bujard, and described in their paper entitled "Independent and tight regulation of transcriptional units in Escherichia coli via the LacR/O, the TetR/O and AraC/I1-I2 regulatory elements" (PMID: 9092630).
Proper citation: RRID:Addgene_58199 Copy
Species: Synthetic
Genetic Insert: U6.gRNAS1 cassette
Vector Backbone Description: Backbone Size:6930; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58252 Copy
Species: Synthetic
Genetic Insert: U6.gRNAGFP cassette
Vector Backbone Description: Backbone Size:7043; Vector Backbone:pBR322-derived pAdEasy plasmid; Vector Types:Mammalian Expression, Adenoviral, CRISPR; Bacterial Resistance:Kanamycin
Defining Citation: PMID:24870050
Comments:
Proper citation: RRID:Addgene_58255 Copy
Species: Homo sapiens
Genetic Insert: ZO1
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58147 Copy
Species: Homo sapiens
Genetic Insert: Actin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:pEGFP; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58149 Copy
Species: Homo sapiens
Genetic Insert: Zyxin
Vector Backbone Description: Backbone Size:4750; Vector Backbone:tdTomato; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Defining Citation: PMID:18057027
Comments: . Excitation = 554; Emission = 581
Proper citation: RRID:Addgene_58148 Copy
Vector Backbone Description: Vector Backbone:Unknown; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: FRET Indicator - Live and Fixed Cells.
Proper citation: RRID:Addgene_58158 Copy
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