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URL: http://www.wormbase.org/db/get?name=WBStrain00051786
Proper Citation: RRID:WB-STRAIN:WBStrain00051786
Description: Caenorhabditis elegans with name ieSi57 II; daf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III. from WB.
Species: Caenorhabditis elegans
Synonyms: ieSi57 II; daf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III.
Notes: ieSi57 [eft-3p::TIR1::mRuby::unc-54 3' UTR + Cbr-unc-119(+)] II. Degron and mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. A single copy transgene was inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the soma. This strain can be used for auxin-inducible degradation (AID) of DAF-2 protein in somatic tissues. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https:|"Made_by: Yan-Ping Zhang, Wen-Hong Zhang"
Affected Gene: WBGene00000898(daf-2)|WBGene00006843(unc-119)
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Source: Integrated Animals
Source Database: WormBase (WB)