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URL: http://www.wormbase.org/db/get?name=WBStrain00051694
Proper Citation: RRID:WB-STRAIN:WBStrain00051694
Description: Caenorhabditis elegans with name hsp-4(utx39[hsp-4::mNG::3xFlag]) II. from WB.
Species: Caenorhabditis elegans
Synonyms: hsp-4(utx39[hsp-4::mNG::3xFlag]) II.
Notes: C-terminal tag of HSP-4 via CRISPR/Cas9 knock-in of mNeonGreen at hsp-4 locus. Insertion verified by PCR and fluorescence. Left flank: 5' TCGGCCGGAGGACAAGGAGAACAAGCTTCTGAGGAGCCATCGGAGGATCATGATGAACTG 3' (1 silent mutation); Right flank: 5' TAAaatattaattgccttcaactacttgct 3'; sgRNA: CGTCTCCAAACTTTACTCGG; Cas9/sgRNA plasmid: pGLOW44; mNG^SEC^3xFlag plasmid: pGLOW61; SEC insertion allele strain: GLW46.|"Made_by: Quincy Martin (Glow Worms '21)"
Affected Gene: WBGene00002008(hsp-4)
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Source: Integrated Animals
Source Database: WormBase (WB)
