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URL: http://www.wormbase.org/db/get?name=WBStrain00051690
Proper Citation: RRID:WB-STRAIN:WBStrain00051690
Description: Caenorhabditis elegans with name ccdc-47(utx31[mNG::3xFlag::ccdc-47]) III. from WB.
Species: Caenorhabditis elegans
Synonyms: ccdc-47(utx31[mNG::3xFlag::ccdc-47]) III.
Notes: Made_by: Anita Rhodes (Glow Worms '20)|"N-terminal tag of CCDC-47 via CRISPR/Cas9 knock-in of mNeonGreen at ccdc-47 locus. Insertion verified by PCR, Sanger sequencing, and fluorescence. Left flank: 5' gttaaatcactcaatttcgggtcgttcacc 3'; Right flank: 5' ATGAAGATAGTATGGATTTTCCTAATATTC 3' (3 silent mutations); sgRNA: cgttcaccATGAAAATCGTC; Cas9/sgRNA plasmid: pGLOW13; mNG^SEC^3xFlag plasmid: pGLOW17; SEC insertion allele strain (balanced): GLW38."
Affected Gene: WBGene00014204(ccdc-47)
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No rating or validation information has been found for ccdc-47(utx31[mNG::3xFlag::ccdc-47]) III..
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Source: Integrated Animals
Source Database: WormBase (WB)
