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URL: http://www.wormbase.org/db/get?name=WBStrain00051688
Proper Citation: RRID:WB-STRAIN:WBStrain00051688
Description: Caenorhabditis elegans with name T28D6.6(utx25[T28D6.6::mNG::3xFlag]) III. from WB.
Species: Caenorhabditis elegans
Synonyms: T28D6.6(utx25[T28D6.6::mNG::3xFlag]) III.
Notes: Made_by: Skye Waterland (Glow Worms '20)|"Superficially wild type. C-terminal tag of T28D6.6 via CRISPR/Cas9 knock-in of mNeonGreen at T28D6.6 locus. Insertion verified by PCR and fluorescence. Left flank: 5' gtcgcaaataatggttttttttccagAGTC 3'; Right flank: 5' TAAgctgaaattcccgtgcttctcgtcttc 3'; sgRNA: gggaatttcagcTTAGACTc; Cas9/sgRNA plasmid: pGLOW2; mNG^SEC^3xFlag plasmid: pGLOW42; SEC insertion allele strain: GLW32. Reference: Huang et al. 2021. Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans. 2021 Sep 16;2021:10.17912/micropub.biology.000460. doi: 10.17912/micropub.biology.000460. eCollection 2021."
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Source: Integrated Animals
Source Database: WormBase (WB)
