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URL: http://www.wormbase.org/db/get?name=WBStrain00034466
Proper Citation: RRID:WB-STRAIN:WBStrain00034466
Description: Caenorhabditis elegans with name ubc-9(iow31[3xFLAG::ubc-9]) IV/nT1[qIs51] (IV;V) from WB.
Species: Caenorhabditis elegans
Synonyms: ubc-9(iow31[3xFLAG::ubc-9]) IV/nT1[qIs51] (IV;V)
Notes: Heterozygotes are wild type with pharyngeal-expressed GFP, and segregate wild type GFP+ heterozygotes, arrested nT1[qIs51] homozygotes, and viable non-GFP ubc-9(iow31[3xFLAG::ubc-9]) homozygotes. ubc-9(iow31[3xFLAG::ubc-9]) homozygotes produce 100% dead embryos, but cytologically they have normal germline (examined by DAPI and RAD-51 staining), suggesting that 3xFLAG::ubc-9 is functional in the gonad but not in the non-maternally rescued embryo. Maintain the strain by picking GFP+ worms. 3XFLAG bas added by CRISPR/Cas9. Synonymous point mutation where included in the repair template (positions 30, 33 and 36 of coding sequence). Reference: Reichman R, et al. Genetics. 2018 Apr;208(4):1421-1441.|"Made_by: Rachel Reichman"|"No longer available from the CGC"
Affected Gene: WBGene00006706(ubc-9)
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Source: Integrated Animals
Source Database: WormBase (WB)
