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URL: http://www.wormbase.org/db/get?name=WBStrain00000062
Proper Citation: RRID:WB-STRAIN:WBStrain00000062
Description: Caenorhabditis elegans with name zen-4(cle10) IV. from WB.
Species: Caenorhabditis elegans
Synonyms: zen-4(cle10) IV.
Notes: Supplementary_genotype zen-4(cle10) IV|"Temperature-sensitive embryonic-lethal mutant. Maintain at 15C. Shift L4s to 25C overnight to observe mutant phenotype of embryos produced by adults. Mutants lack a central spindle during early embryonic mitosis and exhibits a late cytokinesis defect (cleavage furrows regress after ingressing in nearly to the center of dividing embryonic cells). This strain can be used for CRISPR-Cas9 co-conversion. There is a causal mis-sense mutation present in zen-4(cle10), GAC to AAC (D520N), and two silent mutations. One silent mutation is a CGA to CGG mutation at codon 523 that creates a recognition site for a Cas9 guide RNA, in order to use zen-4(cle10ts) as a CRISPR/Cas9 co-conversion marker. The other silent mutation is a GCA to GCT mutation at codon 519 that introduces an AluI site for RFLP analysis. Reference: Farboud B, et al. Genetics Early online November 30, 2018; https:"
Affected Gene: WBGene00006974(zen-4)
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Source: Integrated Animals
Source Database: WormBase (WB)
