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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=408364982
Proper Citation: RRID:RGD_408364982
Description: Rattus norvegicus with name WI-C1ql3em1Lian from RGD.
Species: Rattus norvegicus
Notes: The C1ql3 knout rats were established using CRISPR/cas9 method. The exon 1 of C1ql3 was targeted with two sgRNAs of (TCATC CTC ATC CCG GTG CTGG) and (AAGGT GCT GAC AAG AGG GAGG), which, respectively, targeted on the 5′ end and the 3′ end of exon 1.Wistar embryos born from Sprague Dawley pseudo-pregnant female were genotyped by polymerase chain reaction (PCR) with two upstream primers of (5′-TCCAAAAG CAG ACA AGA GGATC-3′ and 5′-CTACTTCT TCA CCT ACC ACG TCCTG-3′) and one downstream primer (5′-GGCTTCTG AAA CCT TAT ACA TTCTCG-3′). This mutant carried a 631-bp deletion resulting a premature stop at 61 bp of the open reading frame. National Human Disease Animal Model Resource Center, Beijing, China (https://namr.org.cn/Ldesc/1_1074)
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
