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URL: https://rgd.mcw.edu/rgdweb/report/strain/main.html?id=155269039
Proper Citation: RRID:RGD_155269039
Description: Rattus norvegicus with name WI;WDB-ROSA26tm1(H2B-tdTomato)/Nips from RGD.
Species: Rattus norvegicus
Notes: PCR products of the human H2BC11 (H2B-6), tdTomato, splice acceptor sequence, and IRES-Neor-SV40pA were inserted into the NheI site of prROSA26-1 with an in-fusion cloning kit. The final tdTomato-H2B-6 targeting vector was linearized by SalI digestion. The vector was introduced into WDB/Nips-ES1/Nips (RGD ID:10054010) embryonic stem cells by electroporation. Targeted ES cells were injected into Crlj:WI (RGD ID: 2312504) blastocysts to produce chimeric rats. The chimeric rats were crossed with Crlj:WI rats to produce heterozygous founder rats.These rat strains are being maintained by crossing the founder rats with Crlj:WI rats. ROSA26 is a synonym for rat Thumpd3-as1 (RGD:6491660) and is used as an official symbol for rat strain nomenclature. Section of Mammalian Transgenesis Center for Genetic Analysis of Behavior, National Institute for Physiological Sciences, Okazaki Aichi, JAPAN
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No rating or validation information has been found for WI;WDB-ROSA26tm1(H2B-tdTomato)/Nips.
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Source: Integrated Animals
Source Database: Rat Genome Database (RGD)
