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URL: http://www.addgene.org/16298
Proper Citation: RRID:Addgene_16298
Insert Name: Axin
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10072781
Vector Backbone Description: Backbone Size:4352; Vector Backbone:pCS2+MT; Vector Types:Vertebrate expression; Bacterial Resistance:Ampicillin
Comments: For sense mRNA, linearize with NotI and transcribe with SP6. For antisense probe, cut with Pst1, remove 3' overhang, and transcribe with T7. NcoI digest cuts out a 2333 bp fragment.
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Source: Addgene