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URL: http://www.addgene.org/52053
Proper Citation: RRID:Addgene_52053
Insert Name: sfGFP
Organism: Synthetic
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2870; Vector Backbone:pCRT7/NT-TOPO; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: To clone new genes for your proteins of interest into the pCRT7 NT vector provided, use 5’KpnI--‐3’HindIII restriction sites. See Lutz and Bujard 1997 (PMID 9092630) for information on the tetR/pLtetO promoter system used for inducible expression in the pCRT7 NT vector. The phosphoproteins (or sfGFP) expressed via the pCRT7 pLtetO/tetR plasmid is induced with 100 ng/mL anhydrotetracycline.
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No rating or validation information has been found for pCRT7 NT Topo tetR/pLtetO Amp‐WT sfGFP.
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Source: Addgene
