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URL: http://www.addgene.org/51394
Proper Citation: RRID:Addgene_51394
Insert Name: Trim25
Organism: Mus musculus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:23912277
Vector Backbone Description: Backbone Marker:Clontech (modified); Vector Backbone:pLVX; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Ampicillin
Comments: AK154543 (except for K238E (SNP)). For vector modification, we first inserted new multi cloning sites (MCS) between EcoRI and BamHI sites of pLVX-EF1a-AcGFP1-N1 (Clontech, 631983). Next, we removed coding sequences of AcGFP from the above vector by using XbaI sites, and inserted new sequences comprised of Kozak-ATG-3XMyc between BamHI and EcoRI sites.
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Source: Addgene
