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URL: http://www.addgene.org/46888
Proper Citation: RRID:Addgene_46888
Bacterial Resistance: Erythromycin
Defining Citation: PMID:10964628
Vector Backbone Description: Backbone Size:8539; Vector Backbone:pMSP3545; Vector Types:Bacterial Expression, Nisin-inducible expression; Gram-positive Bacterial Shuttle Vector; Bacterial Resistance:Erythromycin
Comments: This plasmid utilizes the NIsin Controlled Expression (NICE) system. NICE® is a registered trademark of NIZO food research BV, P.O.Box 20, 6710 BA Ede, The Netherlands. pMSP3545 contains the Nisin-inducible PnisA promoter, and the pAMB1 replicon for expression in gram-positive bacteria. It also contains a unique NcoI cleavage site immediately downstream of the nisA ribosomal binding sequence, which may be used for translational fusions, and a rho-independent terminator between the XbaI and XhoI sites of the polylinker. For protein expression in E. faecalis, induce with 10-25 ng/mL nisin (Sigma, N-5764). See associated article for instructions on how to make nisin solution. Addgene's quality control sequencing finds discrepancies with the depositor's provided sequence, but they are not thought to affect plasmid function.
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Source: Addgene
