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URL: http://www.addgene.org/42875
Proper Citation: RRID:Addgene_42875
Insert Name: CRISPR-BsaI
Bacterial Resistance: Kanamycin
Defining Citation: PMID:23360965
Vector Backbone Description: Backbone Size:2254; Vector Backbone:pZE21-MCS1; Vector Types:Bacterial Expression, CRISPR, E.coli; Bacterial Resistance:Kanamycin
Comments: This plasmid is also known as pDB129. This plasmid contains a duplicated spacer insertion site (4 BsaI sites instead of 2). Please note that these extra sites are eliminated upon treatment with BsaI, and the depositing lab has used this version extensively without issue. Addgene has found that versions of the plasmid with and without the duplicated spacer are present, both are predicted to perform similarly. The HME63 strain is available from the from the Court Lab: http://redrecombineering.ncifcrf.gov/strains.html For more information on Marraffini Lab CRISPR Plasmids please refer to: http://www.addgene.org/crispr/marraffini/
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Source: Addgene
