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URL: http://www.addgene.org/41726
Proper Citation: RRID:Addgene_41726
Insert Name: 4xCSL binding sites
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11518718
Vector Backbone Description: Backbone Marker:Dr. D. Towler (Washington University, St. Louis); Vector Backbone:RSV-TATA pGL2; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: The 4xCSL-luciferase reporter was constructed from the CBF1/pGL2-GLO TATA CAT plasmid, a gift from Dr. S. Speck. A fragment containing the multimerized high affinity CSL sites (4× CGTGGGAA) was excised by BamHI digest and ligated into a BglII/BamHI-digested RSV-TATA pGL2 vector, a gift from Dr. D. Towler. This modified vector has a TATA box from Rous sarcoma virus inserted into the pGL2-basic vector (Promega) to reduce the basal luciferase activity. Construct was sequenced for verification. Alternate plasmid name: 4xCBS-luciferase
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Source: Addgene
