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URL: http://www.addgene.org/36423
Proper Citation: RRID:Addgene_36423
Insert Name: BCR
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:21310073
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:5400; Vector Backbone:pCDNA3.1/hisB; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: To generate Xpress-tagged Bcr del oligo, we isolated the N-terminal end of Bcr with deletion of amino acid residues 2-63 from a previously described 474 bp subclone by digestion with Eag I x Xho I.The 3.5 kb 3’ end of BCR was isolated from a cDNA clone in pSK (B1/SK) by digestion with Xho I x Xba I. These two fragments were combined in pcDNA3.1/HisB digested with Not I x Xba. Construct has a deletion of amino acid residues 2-63. The first amino acid residues are M- AAA- KEKKSY.... The AAA originate from the pSL1180 polylinker, The "KEKK.." are Bcr amino acids. Constructed by Sun-ju Yi.
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Source: Addgene
