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URL: http://www.addgene.org/35413
Proper Citation: RRID:Addgene_35413
Insert Name: Rnf17l
Organism: Mus musculus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:16093322
Vector Backbone Description: Backbone Marker:Clontech; Backbone Size:4731; Vector Backbone:pEGFP-C1; Vector Types:Mammalian Expression; Bacterial Resistance:Kanamycin
Comments: Based on previously reported Rnf17 cDNA sequences, Rnf17-specific primers for 3'RACE were designed to obtain full-length cDNA sequences for Rnf17s and Rnf17l (Wang et al., 2001; Yin et al., 1999). By using 3'RACE (rapid amplification of cDNA ends), the depositors amplified, subcloned and sequenced Rnf17 cDNA fragments from bulk mouse testis Marathon-Ready cDNAs (BD Biosciences). The composite cDNA sequences were deposited in GenBank under the following accession numbers: Rnf17s, AY854010; Rnf17l, AY854011. Rnf17 long transcript cDNA was cloned in frame with EGFP into pEGFP-C1 with HindIII/SmaI. (Genbank: AY854011)
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Source: Addgene
