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URL: http://www.addgene.org/35203
Proper Citation: RRID:Addgene_35203
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:22848718
Vector Backbone Description: Backbone Marker:Ji-Wu Wang and Brian McCabe; Backbone Size:11976; Vector Backbone:pBID-UASC-GRM; Vector Types:Drosophila Transgene Expression; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Generation of ϕC31 transgenic Drosophila for GAL4 driven expression of myc tagRFP (tRFP) fusion transgenes. Gateway cloning cassette is flanked by gypsy insulator sequences to allow uniform transgene expression between insertion sites. 10 UAS binding sites, Drosophila Synthetic Core promoter (DSCP). myc-tRFP is fused to the Cterminus of genes introduced by gateway. Transgene selection using white gene. loxP site facilitates elimination of transgene markers via Cre recombinase-mediated excision with ZH-attP landing sites. Ampicillin resistant. Chloramphenicol resistant for amplification.
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Source: Addgene
