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URL: http://www.addgene.org/29426
Proper Citation: RRID:Addgene_29426
Insert Name: Venus-5-Venus-5-Venus-5-Venus-5-Venus
Bacterial Resistance: Kanamycin
Defining Citation: PMID:19339497
Vector Backbone Description: Backbone Size:3984; Vector Backbone:pEGFP C1; Vector Types:Mammalian Expression, Anisotropy and brightness standard; Bacterial Resistance:Kanamycin
Comments: Anisotropy standard and brightness standard The 1st Venus can be excised using a NheI-BSP E1 double digest; the 2nd Venus by BSP E1-BglII; the 3rd Venus by BglII-EcoRI; the 4th Venus by EcoRI-EcoRV; the last (5th) Venus by SalI-BamHI. The full insert can be released by a NheI-BamHI double-digest. Due to the multiple Venus repeats in this plasmid, Addgene is unable to confirm the entire insert by sequencing. Please see Addgene's diagnostic digest for this plasmid by clicking the Notes from Addgene link above.
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Source: Addgene
