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URL: http://www.addgene.org/24588
Proper Citation: RRID:Addgene_24588
Insert Name: Gateway(TM) cassette
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:20305087
Vector Backbone Description: Backbone Size:0; Vector Backbone:pLKO.1; Vector Types:Mammalian Expression, Lentiviral; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: The CMV promoter from the pLJM1 vector (Addgene) was PCR amplified and cloned into pLD-puro-EcVA using NdeI/MluI to generate pLD-puro-CcVA. pLD-puro-EcVA (pLD-puromycin resistance- EF1alpha, C-terminal VA tag) was generated as described in the paper from pLKO.1 VA tag= versatile affinity tag, 3XFlag+2XTev+6XHis+2XStrepIII
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Source: Addgene
