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URL: http://www.addgene.org/24418
Proper Citation: RRID:Addgene_24418
Bacterial Resistance: Chloramphenicol and Ampicillin
Defining Citation: PMID:21375737
Vector Backbone Description: Backbone Marker:Soriano Lab (Addgene Plasmid# 21714); Backbone Size:9846; Vector Backbone:pRosa26-1; Vector Types:Mouse Targeting; Bacterial Resistance:Chloramphenicol and Ampicillin
Comments: Murine Rosa26 targeting vector with R1R2 Gateway sites. Includes a ccdB cassette, Rex-Neo selection marker cassette, and DTA selection cassette. Plasmid was constructed as follows: The pRosa26-1 plasmid (Addgene plasmid number 21714) was digested at the XbaI cloning site and a PCR product containing the attR1-ccdb-attR2 sequences from pDest27 and an additional PacI site was introduced. The Rex-Neo resistance cassette from the αMHC-eGFP-Rex-Neo lentivirus plasmid was PCR-cloned into the PacI site to allow for antibiotic selection in eukaryotic cells with G418
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No rating or validation information has been found for pRosa26 R1-ccdB-R2 RexNeo PI-SceI.
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Source: Addgene
