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URL: http://www.addgene.org/218505
Proper Citation: RRID:Addgene_218505
Insert Name: lacI
Organism: Escherichia coli
Bacterial Resistance: Erythromycin
Defining Citation: PMID:38911550
Vector Backbone Description: Vector Backbone:p7INT; Vector Types:Bacterial Expression; Bacterial Resistance:Erythromycin
Comments: This plasmid enables the constitutive expression of LacI in S. pyogenes. It can be used for inducible gene expression in combination with the Plac promoter variants described in the associated publication. Resource information: The Pveg promoter originates from the Bacillus subtilis PY79 genome. The lacI repressor is derived from pPEPY-PF6_lacI (Plasmid #85589) and originally codon-optimised for Streptococcus pneumoniae. However, codon usage is quite similar between S. pyogenes and S. pneumoniae. p7INT was a gift from Prof. Michael Federle (University of Illinois Chicago, USA). The p7INT plasmid integrates into the 3' end of the tmRNA locus in several S. pyogenes strains. It was originally described in: McShan, W.M., McLaughlin, R.E., Nordstrand, A. et al. Vectors containing streptococcal bacteriophage integrases for site-specific gene insertion. Methods Cell Sci 20, 51–57 (1998). https://doi.org/10.1023/A:1009773309163 Please download the file linked below to obtain a well annotated map of the plasmid.
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Source: Addgene
