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URL: http://www.addgene.org/179294
Proper Citation: RRID:Addgene_179294
Insert Name: G0S2
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34260600
Vector Backbone Description: Backbone Marker:New England Biolabs; Backbone Size:5676; Vector Backbone:pMAL-c5E; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Protein sequence is the mature maltose binding protein (identical to aa 1-367 of accession no. 6K7E_A) + NSSSNNNNNNNNNNLG + enterokinase cleavage site (DDDDK*; * = cleavage site) + VPH + human G0S2 (G0/G1 switch protein 2; accession number NP_056529.1) + L. Confers ampicillin-resistance. This plasmid has been referred to as MBP g0s2, MBP-g0s2, g0s2-noHis_pMAL-c5E, or pHis6-MBP-g0s2. There is no His-tag in the expressed protein sequence. Backbone vector is pMAL-c5E. Construct design by James Zook. Publication: Moran et al 2021 PLoS One 16, e0249164, https://doi.org/10.1371/journal.pone.0249164
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Source: Addgene
