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URL: http://www.addgene.org/179143
Proper Citation: RRID:Addgene_179143
Insert Name: Sec61b-V5-TurboID
Organism: Synthetic
Bacterial Resistance: Kanamycin
Defining Citation: PMID:34471136
Vector Backbone Description: Backbone Marker:Tong-Chuan He laboratory; Backbone Size:35725; Vector Backbone:pAdTrack-CMV, pAdEasy-1; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: Sec61b-V5-TurboID_pcDNA5 is Addgene plasmid # 166971. To generate adenovirus, digest with PacI and perform transfection to HEK293 cells according to Luo et al (PMID:17546019) procedure #14. Please note: The plasmid contains a 1650bp insertion just downstream of the Left Arm compared to the depositor's provided sequence. This difference is not known to affect plasmid function.
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Source: Addgene
