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URL: http://www.addgene.org/172217
Proper Citation: RRID:Addgene_172217
Insert Name: Envelope protein of Gibbon Ape Leukemia Virus (codon-optimized version)
Organism: Gibbon Ape Leukemia Virus
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34452336
Vector Backbone Description: Backbone Size:4816; Vector Backbone:phCMV; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: Please note that significantly lower amounts of this codon optimized plasmid are required for efficient packaging. Depending on the vector type and protocol used so far, optimal titers are achieved with 8 to 30 times less plasmid compared to non-codon optimized GALV env. We suggest to start with 0.02 µg per 1x10^6 293T packaging cells (resulting in about 0.1 µg phCMV-coGALVenv-C4070A plasmid per 10 cm cell culture dish). Full details are given in the publication.
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Source: Addgene
