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URL: http://www.addgene.org/17157
Proper Citation: RRID:Addgene_17157
Insert Name: siamois promoter
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Defining Citation: PMID:9308964
Vector Backbone Description: Backbone Marker:promega; Backbone Size:4818; Vector Backbone:pGL3-Basic; Vector Types:Luciferase; Bacterial Resistance:Ampicillin
Comments: Plasmid History: Constructed by David Kimelman, 6/6/96 XVII p. 98. Notes for Use: The -3.0kb-siamois promoter was directionally cloned into the KpnI and BglII sites of the luciferase reporter vector pGL3B (Promega) upstream of the luciferase ATG. The -0.8kb promoter construct was generated by digesting the -3.0kb/pGL3B construct with KpnI and at an internal EcoRI site to remove 2.2 kb of intervening sequence, filling in with DNA polymerase I large fragment and religating. The Kpn site was lost but the R1 site was regenerated in the ligation. The size of the insert is approximate. Citation: Brannon et al., Gene and Development 11:2359-2370, 1997.
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Source: Addgene
