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URL: http://www.addgene.org/16987
Proper Citation: RRID:Addgene_16987
Insert Name: Xwnt-5A
Organism: Xenopus laevis
Bacterial Resistance: Ampicillin
Vector Backbone Description: Backbone Size:2900; Vector Backbone:pGem; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Insert: PCR clone with wnt primers. Insert size: 380 bp. Probe cut with BamH1 and transcribe with SP6. Plasmid History: Constructed by Jan Christian and Randall Moon. Note for Use: Cut with BamH1, transcribe with SP6, yielding 165 nt probe, 142 nt protected fragment. Hybridize 45 degrees C with 50,000 cpm probe (higher probe yields unacceptable background). Run 8% sequencing gel 1.5 hours.
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Source: Addgene
