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URL: http://www.addgene.org/169696
Proper Citation: RRID:Addgene_169696
Insert Name: 1xHA epitope tag adjacent to FRT-flanked cat (CamR) gene
Organism: E.coli and partially synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:11742086
Vector Backbone Description: Backbone Marker:Uzzau et al (PubMed 11742086). Note, pSU311 was initially derived from pGP704 (Miller & Mekalanos; PubMed 2836362); Backbone Size:1932; Vector Backbone:pSU311; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: The 1xHA-FRT-cat-FRT segment is amplified by PCR with primers ending with the sequences 5'...TATCCGTATGATGTTCCTGAT-3' (Forward primer) and 5'...CATATGAATATCCTCCTTAG-3' (Reverse primer) and carrying 40 nt 5' extensions identical to the sequence immediately preceding the stop codon of the targeted gene (Fw primer) and to a region downstream from it (Rv primer). The amplified fragment is used for DNA recombineering in a strain expressing the Lambda Red operon.
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Source: Addgene
