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URL: http://www.addgene.org/169234
Proper Citation: RRID:Addgene_169234
Insert Name: 6xHis mCherry F30-Broccoli
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:35057626
Vector Backbone Description: Backbone Marker:Invitrogen; Backbone Size:2665; Vector Backbone:pEXP5-NT; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Plasmid used for monitoring transcription and translation dynamics. Broccoli aptamer provides a fluorescent signal with DFHBI for mRNA level and mCherry provides fluorescent signal for protein level. Design and sequence of the Broccoli aptamer is from Filonov et al. (2015, doi: 10.1016/j.chembiol.2015.04.018).
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Source: Addgene