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URL: http://www.addgene.org/169159
Proper Citation: RRID:Addgene_169159
Insert Name: 3xFlag-nsp5CS[VATLQ]-nsp14
Organism: SARS-CoV-2
Bacterial Resistance: Ampicillin
Defining Citation: PMID:34198326
Vector Backbone Description: Vector Backbone:pET11a; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: We have included the predicted cleavage site for nsp5 found between nsp13 and nsp14 (VATLQ) between the 3xFlag tag and nsp14 in this construct, which should allow a purified nsp5 protease to cleave-out the 3xFlag tag similarly to what might happen in cells.
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Source: Addgene
