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URL: http://www.addgene.org/16576
Proper Citation: RRID:Addgene_16576
Insert Name: cAPC
Organism: Homo sapiens
Bacterial Resistance: Kanamycin
Defining Citation: PMID:10749138
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:0; Vector Backbone:pShuttle / pAdTrack ?; Vector Types:Mammalian Expression, Adenoviral; Bacterial Resistance:Kanamycin
Comments: The cAPC containing amino acids 958-2075 (nucleotides 2890–6240) was isolated from pCMV-APC by BglII digestion. This fragment was subcloned into the pEGFP-C1 (Clontech). The cassette containing the EGFP-tagged cAPC was further subcloned into the shuttle vector (pShuttle) using Apal I and Mlu I sites.
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Source: Addgene