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URL: http://www.addgene.org/16566
Proper Citation: RRID:Addgene_16566
Insert Name: c-MYC binding sites
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:10688915
Vector Backbone Description: Backbone Marker:Vogelstein Lab; Backbone Size:4867; Vector Backbone:pBV-Luc; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: Different combinations of oligonucleotide pairs (A+B, A+D, C+B, C+D) were annealed and converted to double-stranded fragments through one PCR cycle. These promoter fragments were subcloned into pBV-luc. Further polymerase-derived mutants were identified while sequencing the reporter constructs.
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Source: Addgene
