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URL: http://www.addgene.org/165477
Proper Citation: RRID:Addgene_165477
Bacterial Resistance: Ampicillin
Defining Citation: PMID:24510647
Vector Backbone Description: Backbone Marker:Novagen; Vector Backbone:pET23; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: Primers for LIC cloning: Upstream: add TTAAGAAGGAGATATACT to the 5' end of gene of interest. Downstream: add TGAAAATAGAGGTTTTCGGC to the 3' end of GOI. Detailed cloning method available in the paper Bruni R, Kloss B. High-throughput cloning and expression of integral membrane proteins in Escherichia coli. Curr Protoc Protein Sci. 2013 Nov 5;74:29.6.1-29.6.34.
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Source: Addgene
