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URL: http://www.addgene.org/16443
Proper Citation: RRID:Addgene_16443
Insert Name: p53-binding site (mutant)
Bacterial Resistance: Ampicillin
Defining Citation: PMID:8242752
Vector Backbone Description: Backbone Size:0; Vector Backbone:n/a; Vector Types:Mammalian Expression, Luciferase; Bacterial Resistance:Ampicillin
Comments: MG15-Luc was cloned by inserting a HindIII-EcoRI fragment containing p53-binding elements (Kern et al Science 1992 May 8;256(5058):827-30) into the Hindlll-EcoRI sites of pBluescript II SK(+). A 200 bp EcoRI-BamHI fragment containing the polyoma promoter, and a 2.6 kb SacI luciferase cassette without promoter elements, were then cloned downstream. MG15 contains 15 copies of a mutated p53-binding sequence. Addgene has confirmed the presence of 8 of these copies. Direct repeats of the oligonucleotide MG (5'-CCTTAATGGACTTTAATGG- 3') were used for the p53 nonbinding control sequences.
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Source: Addgene
