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URL: http://www.addgene.org/16419
Proper Citation: RRID:Addgene_16419
Insert Name: GLI
Organism: Homo sapiens
Bacterial Resistance: Ampicillin
Defining Citation: PMID:2832761
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript KS; Vector Types:Bacterial Expression; Bacterial Resistance:Ampicillin
Comments: GlI K12 contains the entire coding region of GLI (-79 to 3522) cloned into the EcoRI site of pBluescript KS. The GLI cDNA contains an internal EcoRI site so the insert should be excised by cleavage with HindIII (at 5' end of Gli cDNA) and XbaI (at 3' end of Gli cDNA).
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Source: Addgene
