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URL: http://www.addgene.org/16413
Proper Citation: RRID:Addgene_16413
Insert Name: PGK-NeoR & ZeoR
Bacterial Resistance: Ampicillin
Defining Citation: PMID:14704360
Vector Backbone Description: Backbone Marker:Stratagene; Backbone Size:3000; Vector Backbone:pBluescript SK+; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Part of vector system to facilitate and simplify the use of rAAV for creating KOs in human cells. The other vectors for this system, pAAV-MCS, pAAV-RC, and pHelper, can be purchased from Stratagene. The sequence information is the sequence between linkers A and B. See author's map and article for more information. In the author's map, restriction sites for the generation of pNeDaKO fragments used for fusion PCR are shown. A and B refer to linkers A and B; yellow arrows: loxP sites; PGK: phosphoglycerate kinase eukaryotic promoter; Neo: neomycin resistance gene; EM 7: EM7 prokaryotic promoter; Zeo: zeomycin resistance gene; Amp: ampicillin resistance gene.
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Source: Addgene
