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Plasmid Name
RRID:Addgene_137046 RRID Copied  
PDF Report How to cite
RRID:Addgene_137046
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Plasmid Information

URL: http://www.addgene.org/137046

Proper Citation: RRID:Addgene_137046

Insert Name: AAC66635.2

Organism: Borrelia burgdorferi B31

Bacterial Resistance: Kanamycin

Defining Citation: PMID:31772280

Vector Backbone Description: Vector Backbone:pelB-MBP (DNASU access no. EvNO00813783); Vector Types:Bacterial Expression; Bacterial Resistance:Kanamycin

Comments: This plasmid uses the T7lac promoter to express in E. coli the mature (lacking the signal peptide), wild-type protein containing an N-terminal PelB signal peptide + His10 + mature maltose binding protein + TEV protease cleavage site. The gene encoding the protein does not have the same DNA sequence as B. burgdorferi because the gene has been optimized for expression in E. coli.

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Usage and Citation Metrics

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Ratings and Alerts

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Data and Source Information

Source: Addgene