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URL: http://www.addgene.org/129027
Proper Citation: RRID:Addgene_129027
Insert Name: dCas9-huTET1CD, SgRNA cloning site
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:33488615
Vector Backbone Description: Backbone Marker:Feng Zhang (Addgene plasmid # 48138); Vector Backbone:PX458 ; Vector Types:Mammalian Expression; Bacterial Resistance:Ampicillin
Comments: For SgRNA cloning: Caution! BbsI cannot be used for SgRNA cloning in this plasmid, there are BbsI sites in TET1CD. Instead the guide sequence can be cloned into Addgene#48138 and in a second step transferred into this plasmid (for example using PvuI & XbaI). This plasmid was generated by Christopher Kressler.
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Source: Addgene