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URL: http://www.addgene.org/128167
Proper Citation: RRID:Addgene_128167
Insert Name: mTagBFP2-T2A-sfGFP-GSAx9-iCre-ERT2
Organism: Synthetic
Bacterial Resistance: Ampicillin
Defining Citation: PMID:32183906
Vector Backbone Description: Vector Backbone:pCAGGS; Vector Types:Mammalian Expression, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: Linker between sfGFP and iCre-ERT2 is a clean GSAx9 sequence, similar in length to the mutated P2A sequence in the plasmid pCAG-sfGFP-iCre-ERT2 (#125749). The sfGFP fluorescence from the sfGFP-iCre-ERT2 is dim, as described in Nam and Capecchi, 2020 (https://doi.org/10.1186/s13064-020-00139-5) and a Figshare page (https://doi.org/10.6084/m9.figshare.13218437.v35). In short, it is not visible to the eyes, but can be detected with flow cytometry.
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Source: Addgene
