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URL: http://www.addgene.org/122860
Proper Citation: RRID:Addgene_122860
Insert Name: 35S:MS2-VP64:tRBCS
Organism: Synthetic
Bacterial Resistance: Spectinomycin
Defining Citation: PMID:31557222
Vector Backbone Description: Backbone Size:2740; Vector Backbone:pGreenII; Vector Types:Golden Gate compatible plant transformation vector; Bacterial Resistance:Spectinomycin
Comments: Plasmid is an intermediate stage destination vector constructed by Golden Gate cloning using the enzyme BsaI, and containing two adjacent BsaI recognition sites downstream of the MS2 expression cassette for insertion of additional modules. Plasmid Features (listed as bp in full plasmid sequence): 35S promoter = 656-1502bp MS2-VP64 coding sequence = 1542-2195bp RBCS terminator = 2203-2848bp BsaI site #1 = 2883-2888bp BsaI site #2 = 2897-2902bp
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Source: Addgene
