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URL: http://www.addgene.org/11619
Proper Citation: RRID:Addgene_11619
Bacterial Resistance: Ampicillin
Defining Citation: PMID:16550170
Vector Backbone Description: Backbone Marker:N/A; Backbone Size:8500; Vector Backbone:pLL3.7; Vector Types:Mammalian Expression, Lentiviral, RNAi, Cre/Lox; Bacterial Resistance:Ampicillin
Comments: pLB is a modification of pLL3.7. Two genetic elements known to prevent epigenetic silencing were added. A fragment of one antirepressor element (#40) was cloned upstream of the mouse U6 promoter and a scaffold-attached region (SAR) was cloned downstream of GFP. Please see author's map for more detailed information. Note: A single base pair deletion at position 11 of the mouse U6 promoter in this plasmid does not impair the efficacy of this reagent. There is also a base pair insertion upstream of the promoter. The depositing lab has no indication that it functionally impairs pLB.
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Source: Addgene
