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Basic research equipment for RCMI Faculty

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• SciCrunch
• 13 years ago - by Anonymous

The microscopy center is a shared central facility whose primary goal is to promote scientific research by providing training in basic operation of the scanning electron microscope (SEM), the transmission electron microscope (TEM) and the Confocal.RRRRR RRRRR New users of the facility are asked to submit an application then schedule an appointment with the manager to discuss the project or to begin training.

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• SciCrunch
• 13 years ago - by Anonymous

LSR II A State-of-the-Art RCMI Flow Cytometry Core is located in MR613 with full time technical support, provided by the Flow Technician Mr. Jeffrey Walker. Mr. Walker is BD trained and certified in both analysis and sorting and has extensive experience in sorting both lymphocytes and larger epithelial cells, as well as with specialized applications including cell cycle analysis and calcium flux. Mr. Walker provides training for regular users wishing to perform independent analysis, and is responsible for all sorting.

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• SciCrunch
• 13 years ago - by Anonymous

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on October 27,2023. Core provides next generation sequencing service. Collaborative effort between the CCRTD and the Ovarian Cancer Institute at Georgia Tech University (OCIGT). CCRTD provides laboratory to prepare libraries and to operate the instrument, while OCIGT contributes bioinformatics support for processing the data and additional services to complement genomics data including hardware, software, and personnel. OCIGT contributes patients sample procurement and complementary technologies including Affymetrix microarray technology and mass spectroscopy analysis.

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• SciCrunch
• 13 years ago - by Anonymous

CCRTD houses several major instruments to support structural studies of biological molecules, cells, modern cell, and molecular visualization applications. We have an Inverted Digitized Microscope (Zeiss) of general use, plus an advanced Fluorescence-enabled Inverted Digitized Microscope with Z-stacking and Live Cell Imaging modules (Zeiss). For advanced applications including In Situ Hybridization (ISH) and Immunohistochemistry (IHC), in addition to Zeiss microscope above we offer Confocal Fluorescent Microscope (Nikon). The latter is currently undergoing a major upgrade of computer system, software, and optics including addition of lasers.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Mouse Long Bone Scans, Mouse Vertebrae Scans. The Harvard School of Dental Medicine under the direction of The Office of Research operates a core facility composed of two micro CT machines.

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• SciCrunch
• 13 years ago - by Anonymous

CCRTD Core Facilities house major equipment for DNA and RNA-based applications. We offer polymerase chain reaction ( PCR ) instruments including Real-Time PCR machine (I-Cycle, Bio-Rad), Liquid Scintillation Counter (Beckman Coulter), Lyophilizer and Freeze-Dryer for biological samples, Cell Porator, high-speed and ultra-high-speed Centrifuges, ultra-sensitive Balances, and other supporting equipment. DNA and RNA quantification can be carried out on Spectrophotometers, both single-cuvette (DU650, Beckman Coulter) and 96-well plate based. DNA and RNA can be visualized, digitized, and quantified using Gel Documentation system (Bio-Rad) or Multi-Mode Imager Typhoon 9410, in fluorescent, luminescent, UV, or visible wavelengths; time-resolved fluorescence option is available on BioTek Plate Imager. For X-Ray imaging, we maintain an Automated X-Ray processor and Dark Room; radioisotope imaging is also supported on Typhoon 9410.

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• SciCrunch
• 13 years ago - by Anonymous

In CCRTD, we provide excellent support for Cell Biology research and advanced cell visualization applications. We have complete facilities for cell growth, handling, and storage. Cell Culture Facility maintains Cell Storage Cryo Tanks with uninterruptable liquid nitrogen supply and two separate Cell Culture rooms fully equipped with eight temperature and CO2-controlled Cell Growth Incubators, two Biological Safety Cabinets, Fluorescence-Activated Cell Sorter (FACS), two late model Inverted Digitized Microscope (Zeiss), one of which is fluorescence-enabled and possesses Z-stacking and live cell imaging capabilities, automated Cell Counter (Nexcelom Bioscience), and refrigerated storage for media and cell culture reagents.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: DNA analysis - Affymetrix GeneChip arrays service, DNA analysis - Illumina infinium whole genome genotyping service, DNA analysis - custom content genotyping service, RNA Analysis - Affymetrix gene expression service, RNA Analysis - Illumina Gene Expression service, RNA Analysis - Illumina whole-genome DASL service. The Genetic Analysis Platform collaborates with scientists at the Broad and across the world to identify and characterize patterns of genetic variation and gene expression. These patterns can yield a deeper understanding of how genetic factors influence disease risk and treatment outcomes in a wide range of human diseases, including type 2 diabetes, Crohn''s disease, schizophrenia, and cancer, among many others. Over the past several years, the platform has been a leading contributor to major projects such as the International Haplotype Map Project, The Cancer Genome Atlas, the 1,000 Genomes Project, the Genes and Environment Initiative, and served as a national genotyping center under a NIH/NCRR program. Genetic Analysis Platform (GAP) scientists are experts in a wide range of genomic technologies and technology platforms, including genotyping, gene expression and directed resequencing, and routinely apply these technologies to studies of both human and non-human samples. In addition, teams of platform researchers work to test out emerging genetic technologies, develop informatics tools and infrastructure to enhance scientific research, and coordinate large-scale projects involving collaborators both within and beyond the Broad.

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• SciCrunch
• 13 years ago - by Anonymous

The BWH-BRI Antibody Core Facility provides high quality, purified monoclonal antibodies to the BWH research community. Because the antibodies are made in-house and require no shipping, they are available at a cost substantially lower than that of commercial vendors. Antibodies are of standardized quality and are available in two convenient aliquots: 500ugs and 10mgs. The Core maintains an inventory of commonly used antibodies, which allows orders to be filled quickly.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: ES cell transfection (electroporation), Thawing/expansion of targeted ES cells, Microinjection of ES cells into blastocysts, Microinjection of DNA constructs into egg pronuclei for generating transgenic mice, Microinjection of BAC DNAs, DNA injection and microdissection, Lentiviral transgenics service, Injection of induced pluripotent stem cells for testing totipotency, Tail biopsy service, Breeding services, Mouse manipulation consultation. The Brigham & Women''s Hospital Transgenic Core Facility provides services for generating transgenic and knockout mice. Established in 1992, the BWH Core Facility has been providing gene targeting and microinjection services using state-of-the-art facilities and equipment. Dr. Lina Du, formerly a plastic surgeon in Beijing and an expert in microvascular surgery, performs both blastocyst and pronuclear injections. She has over 15 years of experience in embryo manipulation and the generation transgenic mice. The Core Facility has all of the equipment necessary for generation of transgenic mice including a Nikon Diaphot microscope equipped with Nomarski Optics and Narishige micromanipulators for microinjections, Nikon surgical microscopes for egg isolation ad transfer, a sutter needle puller and a de Fonbrune microforge. The facility maintains mice necessary for egg donors, egg recipients, and vasectomized males. This is a non-profit facility and charges are based upon anticipated mouse costs, maintenance of mice and equipment, and purchase of necessary surgical supplies and chemicals. The ES cell culture service has been offered for 6 years. Our staff carries out electroporation of targeting vectors into ES cells and provides investigators with DNA to identify ES cells carrying the desired recombination events. Then they expand the appropriate ES Cells for microinjection into blastocysts by the BWH Transgenic Core.

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• SciCrunch
• 13 years ago - by Anonymous

We are equipped for proteomics research including Cell Fractionation on one floor and tabletop Ultracentrifuges (Beckman Coulter) , Fast Protein Liquid Chromatography (FPLC), Two-Dimensional Protein Electrophoresis (Protean II, Biorad), and Gel Documentation Systems (one Bio-Rad and one Fuji Medical). Protein quantification can be done using UV-Vis spectrophotometry ( DU650, Beckman Coulter) or multimode Plate Spectrophotometers in both 96-well and 384-well micro well plate formats. Protein gels can also be visualized, digitized, and documented on Multi-Mode Imager Typhoon 9410.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Immunoassay expertise for several methodologies, Specialty hormone analysis. The Specialty Assay Research Core Lab (CLIA certified laboratory) is an analytical research core designed to provide scientific and analytical services to the Partners and non-Partners research communities. The primary objective of the core is to provide sensitive, cost-effective, and reproducible clinical research assays to investigators for endocrine, diabetes, bone markers, inflammatory and sleep medicine related research in both human and animal clinical research protocols. The core hopes to collaborate with investigators in biomarkers discovery and to offer new tools to the scientific community. The core also serves as a central laboratory for multisite studies. The SARC facility offers tests for any investigators (local or national) or industry, but preference may be given to internal users. Different rates may apply.

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• SciCrunch
• 13 years ago - by Anonymous

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• SciCrunch
• 17 years ago - submitted by Timothy Tsui

Core facility that provides the following services: PSG Procedures, PSG Data Archiving, PSG Training to Staff, PSG Data Processing and Analysis Support. The Sleep & EEG Core within the Division of Sleep Medicine (DSM) provides an integrated infrastructure and knowledge base in support of research projects that use polysomnography (PSG), quantitative EEG analysis and related methodologies. The Core consists of a team of specialists lead by the Core director and a chief PSG technologist. The Core provides support and services in different areas: # It provides basic training and certification in PSG and EEG instrumentation to technicians and investigators, particularly those conducting studies at the Center for Clinical Investigation (CCI) at Brigham and Women?s Hospital. # It acts as liaison between DSM investigators and CCI technical staff, and implements and monitors quality assurance measures. # It carries out standard vigilance state scoring of PSG recordings and different types of waking EEG and electrooculogram analyses. # It carries out spectral analysis of sleep and waking EEG. # It evaluates, acquires, and maintains PSG and EEG equipment used by investigators of the CCI. # It carries out PSG procedures such as sleep screens and multiple sleep latency tests. # It assists investigators in the analysis and interpretation of sleep and EEG data. Over the years, the Sleep & EEG Core has been a central part of many projects funded by NIH, the Air Force Office of Scientific Research (AFOSR), and the National Space Biomedical Research Institute (NSBRI) that had a main focus on the physiology of human sleep-wake regulation. Scientifically, the Core has contributed by providing investigators with important quantitative measures of homeostatic and circadian components of the human sleep-wake regulatory system.

• Resource
• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Sorting or analysis with operator service, Calcium flux measurements service, DNA analysis service, Cell sorting service. <BR/> <BR/>This lab''s mission is to provide high standard cell sorting and analysis services to the research community at the Brigham and Women?s Hospital and the Longwood Medical area.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: DNA sequencing, Multiplex ligation-dependent probe amplification analysis, Capillary electrophoresis. <BR/> <BR/>The DNA Sequencing Core mission is to provide a highly accurate, high quality DNA sequencing service to the BWH community and other users. Our turn-around-time is 36 to 72 hrs.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Fluorescence in situ hybridization. <BR/> <BR/>The CytoGenomics Core provides an invaluable technical resource to the investigators of the BWH, MGH, and affiliated institutions. Services are also available to external academic and commercial laboratories; these should contact us via our website for sample submission and pricing. Cytogenetic studies can provide insight into regions of the genome that are pathogenetic in various neoplasms leading to an understanding of the molecular pathways participating in the biology of cancer. It is appropriate to consider cytogenetics as a fundamental adjunct to a variety of investigations underway, including basic and clinical research. For example, a rather simple cytogenetic analysis of mouse ES cells to determine ploidy prior to injections into blastulas leads to a greater success rate in establishing founders for knock-out and knock-in experiments. The primary chromosomal assignment of a gene by a FISH experiment may lead to correlation of a disease with that gene. Other cytogenetic studies may be important in establishing a diagnosis for correlation with clinical outcome. The advent of molecular probes for FISH analysis has facilitated cytogenetic studies in the mouse, and other model organisms and this Core aggressively implements such technologies. In addition to state-of-the-art analyses for human samples, the BWH CytoGenomics Core also performs routine mouse karyotyping and a variety of other molecular cytogenetic analyses, services not easily obtainable elsewhere.

• Resource
• SciCrunch
• 13 years ago - by Anonymous

Biostatistics support is available to investigators in the Brigham and Women''s and Harvard Medical research community for statistical consulting on issues such as study design and data analysis for manuscripts and grant applications. Resources are limited, so planning ahead is essential. The Biostatistics Consulting Service is part of the Biostatistics Core of the BWH Center for Clinical Investigation (CCI).

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Data acquisition service, Data storage service. <BR/> <BR/>The Research Electronic Data Collection Core can help you capture research data by supporting all aspects of data acquisition, storage and retrieval. The core is designed to significantly improve the efficiency, cost structure and overall quality of research data collection. The Data Collection Core will provide expert services including: study forms design and implementation; training in all aspects of using TELEform software; database construction and exporting / preparing data for statistical applications such as SAS and SPSS; and technical assistance with scanners and computers running electronic form capture process.

• Resource
• SciCrunch
• 13 years ago - by Anonymous