Curator Dashboard

THIS RESOURCE IS NO LONGER IN SERVICE.Documented on July 29,2022. Core to support the implementation of bioinformatics resources for cancer research at Dartmouth. Provides consultation and collaboration for research projects of NCCC members, regular workshops, seminars, services including applied bioinformatics and data mining, computer programming and software engineering, database development and programming and high performance computing and systems administration.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Medium-density speed congenic backcross service, Medium-density genetic background check service, DNA extraction. DartMouse is a not-for-profit core facility funded by the National Institutes of Health''s National Center for Research Resources. The mission of DartMouse is to facilitate the development of congenic mice in support of pre-clinical projects across the United States. Use of DartMouse allows the generation of congenic mice in 5 generations (~1.5 years), versus the 10 generations (~3 years) required by conventional back-crossing. Facility staff provides expert advice on mouse speed congenic development, mouse genetic background analysis, and mouse genetic mapping. Investigators provide us with mouse tail clippings. From these, DartMouse isolates genomic DNA and performs and analyzes complete genome-wide scans. We return data in graphical and spreadsheet formats, and make specific recommendations on breeder selection. We operate an Illumina BeadStation 500. Chips use a 1449 SNP array covering the mouse genome with an average density of <5 cM.

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• SciCrunch
• 13 years ago - by Anonymous

The Molecular Diagnostics Laboratory provides such research services as microRNA expression profiling, human cell line identity verification, mutation detection for clinical research studies, and specimen processing for clinical research studies to investigators at the Dana Farber Cancer Institute, as well as to investigators at other institutions. Consultation on experimental design and assistance with data analysis are also available.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Genome-scale screening of lentiviral human and mouse shRNA libraries. <BR/> <BR/>The RNAi Screening Facility provides investigators with access to RNAi reagents and high-throughput screening technology. Lentiviral-based RNAi constructs against the human and mouse genomes are provided by the RNAi Consortium of the Broad Institute, and can be used to transduce a wide range of target cells, including primary and non-dividing cells. The Facility provides reagents, equipment, and technical expertise to design, optimize, and perform cell-based, arrayed lentiviral RNAi screens. Additionally, lentiviral RNAi constructs against individual genes can be requested.

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• SciCrunch
• 13 years ago - by Anonymous

Core offers services for genomic next-generation sequencing library preparation, sequencing and analysis applications including RNAseq, ChIPseq, ATACseq, CRISPR screening, whole genome methylation profiling, targeted resequencing, single-cell RNAseq, exome sequencing, and more. Performs bioinformatics analysis such as integration of multi-omics datasets or specialized analyses. Genomics core technology platforms include Illumina NovaSeq6000, NextSeq500s, MiSeqs, MiniSeq. High throughput sample preparation is performed on Beckman Coulter Biomek FX and i7 systems. Low throughput samples are prepared by technical staff.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Gene expression profiling service, Single nucleotide polymorphism analysis service, Tiling arrays service. <BR/> <BR/>The Microarray Core provides services for genome-wide analysis of gene expression, nucleotide variation, copy number variation, and chromatin protein binding sites. The core platforms include microarrays and next generation sequencing. Consultation on experimental design, and assistance with data analysis are also available. The Microarray Core carries out gene expression profiling on 3'' arrays and on Exon arrays; genotyping and comparative genomic hybridization both on single nucleotide polymorphism (SNP) arrays, and Chromatin ImmunoPrecipitation localization (ChIP-on-Chip). All services use oligonucleotide microarrays. Biostatistical support to assist in analyzing the data is available. The services of the Core are accessible to DFCI investigators and to investigators outside of DFCI.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Graphics services, All Research Related Photography. <BR/> <BR/>Medical Arts Core DFCI offers complete services for Publications, Presentations and Advertising as follows: Custom/Computer Graphics illustrations, Design and Consulting full productions services, Photoshop training. Posters Productions/Brochure Printing on best selection of paper, canvas material and Backlit poster films. Lamination, LCD announcements creation and posting. Research Related Photography Equipment: * iMAC and PC computers * Posters printer * LCD electronic announcements TV units * Backlit Display units * Laminator * Scanner * Digital camera

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Cell Sorting. <BR/> <BR/>The Flow Cytometry Core Facility provides sterile sorting and analysis of samples in support of research at DFCI. The core has 2 high-speed cell sorters, the BD FACSAria II SORP and BD FACSAria IIu with up to 12-color simultaneous detection utilizing multi-laser platforms. Sample populations can be sterile sorted into a variety of tubes or plates. The BD FACSAria II SORP and BD FACSAria IIu are both equipped with the Automated Cell Deposition Unit (ACDU) for sterile sinlge cell sorting into 96, 384 micro titer plates. The core presently has 2 analyzers, the BD LSRFortessa SORP and BD LSRII with up to 12-color simultaneous detection utilizing multi-laser platforms. The BD LSR II is also equipped with an HTS(high throughput sampler) for analyzing samples directly from 96 well micro titer plates as well as being able to run 5ml tubes. The Facility staff is available to assist researchers with their sorting and analysis needs. Researchers who would like to utilize the analyzers are trained so that they can analyze their experiments independently. The staff will assist with data acquisition and analysis as well as interpretation. They will also assist in experiment design and implementation as well as troubleshooting. Training sessions are available for researchers who would like to run their sorting experiments independently.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Data collection, data entry and analysis, Database design, data management and process tracking, Qualitative data collection, management and analysis. <BR/> <BR/>The Survey and Data Management Core (SDMC) provides efficient, high-quality survey data collection and management services and consultation to support the research needs of investigators and staff throughout the Harvard Medical research community. The Core, housed within the Center for Population Sciences at the Dana-Farber Cancer Institute, is dedicated to rigorous quantitative and qualitative survey data collection and management.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Separation of plasma and serum from blood samples, Mononuclear cell counting and isolation, DNA isolation, 24 hour urine volume and aliquoting, Research specimen processing, Bone marrow aspiration and blood fixation, Research specimen storage. <BR/> <BR/>The Clinical Research Laboratory (CRL) utilizes protocol-driven standard operating procedures for the processing of pharmacokinetics, pharmacodynamic, and biomarker samples. This includes the processing, storage, and tracking of all research specimens to ensure the successful completion of clinical trials associated with patient treatment. The CRL is dedicated to the highest standards of specimen handling to maintain the integrity of data procurement and analysis for clinical trials.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Mass spectrometry service. <BR/> <BR/>The Blais Proteomics Center develops and applies state-of-the-art proteomics, informatics, and related technologies for direct interrogation of protein expression, modification, and function in response to biological perturbation in cell-based models of human cancer and primary tissues. The Blais Proteomics Center serves as a valuable resource to support individual research labs at the Dana-Farber and throughout the surrounding research community. As a key component of Dana-Farber?s Strategic Plan for Research, Blais Proteomics actively participates with other Dana-Farber Strategic Research Centers in large-scale studies, designed to leverage disparate capabilities in pursuit of novel, in-depth, and otherwise unattainable insights into human biology and disease. Consistent with our role as a world-class, center of excellence in proteomics science, members of the Blais Proteomics center contribute to the teaching mission at Harvard Medical School and participate in outreach activities designed to introduce and train young scientists and others who may not otherwise have access to these advanced technologies.

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• SciCrunch
• 13 years ago - by Anonymous

THIS RESOURCE IS NO LONGER IN SERVICE. Documented on October 27, 2023. Core facility that provides the following services: Storage services for -80 degrees C and -190 degrees C in secure facility. <BR/> <BR/>The Biospecimen Repository provides long-term storage of clinical and research material in -80 degrees C and liquid nitrogen freezers located at Dana Farber''s Harbor Campus. Transportation of samples to and from Harbor Campus is provided by the facility for a small fee. Competitive prices are available on per box or per freezer basis in both segregated or non-segregated environments.

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• SciCrunch
• 13 years ago - by Anonymous

The Biocontainment Core Facility supports researchers whose experiments require a Biosafety Level 2 enhanced (BL2+) containment level. The Biocontainment Core adheres to Biosafety Level 3 (BL3) practices. Protective apparel, equipment, and other materials are provided by the core. Two core facilities are available: one is located at the Jimmy Fund building at Dana Farber; the second at The Center for Life Science. Authorized core users may have access to both facilities. Use of the facility is limited to researchers who receive training in BL3 procedures and comply with authorized user requirements. Training is provided by the Biosafety Officer, Core Manager, and authorized users.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Mouse breeding management, Mouse irradiation service, Technical services for laboratory animal experimentation, Veterinary care, Animal care training. <BR/> <BR/>The facility provides services and resources for investigators looking to accomplish animal research objectives. This includes housing mice and rats, purchasing animals, training researchers regarding proper animal care and use, and monitoring the safety of all personnel with laboratory animal contact in conjunction with Dana-Farber?s Environmental Health and Safety and Occupational Health Services. Dana-Farber has established an Institutional Care and Use Committee (IACUC) to oversee the Institute?s animal program, facilities, and projects involving the use of animals. The IACUC serves as the approval body for all protocols involving animal research, and assists faculty, students and staff in upholding Dana-Farber?s commitment to providing the finest care and most humane utilization of laboratory animals. In addition to basic husbandry services, the ARF staff provides technical and veterinary services, mouse breeding management and mouse irradiation.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Reporting and database management service of radiological data for clinical trials, Radiologic exam quality control service. <BR/> <BR/>The mission of the Tumor Imaging Metrics Core (TIMC) is to provide standardized, consistent, longitudinal radiological measurements to evaluate therapeutic response for DF/HCC clinical trials. The TIMC - * Makes reliable, quantitative, longitudinal measurements (such as RECIST, WHO, SWOG, standardized uptake value (SUV) etc.) of lesions from serial MRI, CT, PET, and PET/CT scan images * Presents results of analyses on a password-protected secure web-based report * Provides an independent service, with verifiable measurement of treatment response for patients enrolled in cancer center trials * Serves as a centralized, computerized resource to facilitate efficient internal or external auditing Reliable tracking of changes in lesion size or metabolism is essential for clinical trials that use radiological measurements as surrogate endpoints. The Tumor Imaging Metrics Core provides a service for managing these images, making measurements in a routine, reliable, and centralized way, and making results available to investigators efficiently.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Construction of standard and custom TMA service, Nucleic acid isolation from paraffin embedded tissue service, Immunofluorescent staining service, Access to the Automated Quantitative Analysis (AQUA) system, Tissue microarray consultation service. <BR/> <BR/>The mission of the Tissue Microarray and Imaging Core is to generate tissue microarrays by allowing placement of up to 480 0.6 mm diameter tissue samples into a single standard tissue block, allowing hundreds of tumors and samples to be evaluated on a single slide. The DF/HCC Tissue Microarray & Imaging (TMI) Core is dedicated to the construction and evaluation of high quality TMAs for cancer research. In addition, the Core performs high-throughput isolation of DNA and RNA from formalin-fixed paraffin-embedded tissues. The Core also provides web-based digital pathology services using the Aperio system. These services include slide scanning, image viewing and storage, and quantitative image analysis, which facilitate tissue-based research. Finally, the Core provides access to the Automated Quantitative Analysis (AQUA) system.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Histopathology analysis service, Histopathologic diagnosis training service, Cryosectioning service, Rodent necropsy service, Paraffin sectioning service, Bacteria, fungi, and inclusion bodies histological staining service, Rodent tissue processing, Carbohydrate and mucoproteins histological staining service, Connective tissue and muscle histological staining service, Cytoplasmic granules histological staining service, Fats and lipids histological staining service, Hematologic and nuclear elements histological staining service, Nerve cells and fibers histological staining service, Equipment training service, Equipment access service. <BR/> <BR/>The Rodent Histopathology Core was founded in 1999 as one of the pathology cores within the Dana-Farber/Harvard Cancer Center. The Core was established to provide high quality professional, technical, and educational pathology services. The ultimate goal of the facility has been to support investigator research that leads to the identification of pathologic processes in mice and can be directly translatable to human disease. The core provides an essential and unique service to the many Center members and labs invested in mouse research. While many commercial and academic histology services are available, no other service is available that offers comparable high quality service with rapid turn around time and highly experienced professional supervision. The educational mission of the Core also adds an important dimension.

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• SciCrunch
• 13 years ago - by Anonymous

PSL is a consultative Core with components in each of the five DF/HCC hospitals. We have established a web-based network of autonomous, distributed, searchable databases, which contain de-identified, coded, pathologic information on post-diagnostic, excess human materials (including frozen or paraffin-embedded tissues and bodily fluids). Standardized specimen core dataset includes age, gender, anatomic location, tissue type, and diagnoses. Access to the PSL is through the password-protected Harvard eCommons. Two types of generic PSL uses are envisioned: (1) Center members with eCommons accounts can quickly retrieve aggregated tissue availability information to determine the feasibility of a proposed study. (2) Approved investigators can discover and review materials from suitable, de-identified and coded cases online and then request the samples they want.

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• SciCrunch
• 13 years ago - by Anonymous

Core facility that provides the following services: Protein development/cloning, Western blot service, ELISA service, Flow cytometry service. <BR/> <BR/>The mission of the DF/HCC Monoclonal Antibody Core (MAC) is to produce novel monoclonal antibodies that are directed against antigens of interest to Dana-Farber, the Harvard Cancer Center, and affiliated investigators. These monoclonal antibodies may be useful for basic research, drug discovery and clinical applications including diagnosis, surrogate markers for disease status, response to therapy or drug toxicity. Given the diverse research needs of investigators at DFCI and the Harvard Community, the MAC should strive to support a wide range of antibody requests. A key component of the DF/HCC MAC is the ability to explore and develop new technologies that facilitate generation of monoclonal antibodies. The DF/HCC MAC should be capable of supporting all basic functions of generating, screening, storing and producing monoclonal antibodies.

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• SciCrunch
• 13 years ago - by Anonymous

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• SciCrunch
• 13 years ago - submitted by Andrea Stagg